1995 Fiscal Year Final Research Report Summary
Biosynthesis and processing of cathepsins B,H and L in carp macrophages
| Project/Area Number |
05660234
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
HARA Kenji Nagasaki University, Faculty of fisheries, Associate professor., 水産学部, 助教授 (10039737)
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| Co-Investigator(Kenkyū-buntansha) |
TACHIBANA Katsuyasu Nagasaki University, Faculty of fisheries, Associate Professor., 水産学部, 助教授 (20171712)
TSUCHIMOTO Mutsuyoshi Nagasaki University, Faculty of fisheries, Professor., 水産学部, 教授 (20080525)
ISHIHARA Tadashi Nagasaki University, Faculty of fisheries, Professor., 水産学部, 教授 (40039722)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Fish Cathepsins / Biosynthesis / Processing / Macrophage / Pulse-chase experiment |
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| Research Abstract |
Cathepsins B,H,and L were purified from carp hepato-pancreas or muscle to prepare their antibodies.
15 resudures of N-terminal amino acid sequences of cathepsin L were determined. Polyclonal antibodies of cathepsins B and L,and monoclonal antibody of cathepsin L were prepared by using purified enzymes.
We established the method of preparation of macrophage monolayr from carp peritoneal exudate cells.
The intracellular processing and release of cathepsins B and L by carp peritoneal macrophages were investigated by pulse-chase experiments. Newry synthesized procathepsin B (39 kDa) after 15 min labeling was processed to the mature, single-chain enzymc (29 kDa) within 1 h. However two-chain form of cathepsin B was not detected during 24 h.
Though processing of cathepsin L was not clarified, cathepsi L was synthesized as procathepsin L of 39 kDa.
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