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1995 Fiscal Year Final Research Report Summary

Role of cell surface carbohydrates on the development of mammalian embryos in vitro

Research Project

Project/Area Number 05660327
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YAMADA Masayasu  Kyoto Univ., Faculty of Agri., Associate Professor, 農学部, 助教授 (10243073)

Co-Investigator(Kenkyū-buntansha) HOSOI Yoshihiko  Kinki Univ., Res.Inst.of Bio-Oriented Sci.and Tech., Instructor, 生物理工学部, 講師 (70192739)
MINAMI Naojiro  Kyoto Univ., Faculty of Agri., Researcher, 農学部, 助手 (30212236)
UTSUMI Kyozo  Kyoto Univ., Faculty of Agri., Professor, 農学部, 教授 (90033266)
Project Period (FY) 1993 – 1995
Keywordspreimplantation / embryo / in vitro development / PHA-P lectin / compaction / N-linled carbohydrates / oviduct extracts
Research Abstract

The developmental block at the 2-cell stage in rat embryos cultured in vitro has recently been overcome by deleting phosphate and glucose from culture medium. However, the problem was still remained that early 1-cell embryos were less able to develop in vitro to the blastocyst stage than 2-cell embryos. To improve the culture conditions for the 1-cell embryos, we first examined the effect of PHA-P lectin, which is known to induce in vitro maturation of mouse oocytes. As the results, it was found that PHA-P,especially E_4 subunit promoted the developmental ability of rat 1-cell embryos to blastocysts in vitro, suggesting that the action of PHA-P is specific and a modification of cell surface carbohydrate moieties of the embryos. Furthermore, we found that development of rat 1-cell embryos to blastocysts was promoted with macromolecules in rat oviduct extracts, whose molecular mass was about 70kDa.
Concerning the study on the involvement of Cell surface carbohydrates in compaction in the mouse embryos at the morula stage, it was revealed that cell surface N-linked carbohydrates that were specifically cleaved by N-glycanase participated greatly in the compaction process.

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Yamada, M.: "Effects of Nacl and KH_2PO_4 on in vitro development of 1-cell rat embryos in a protein-free defined medium." J. Assist, Reprod. Genet.10. 164 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minami, N.: "Role of the oviduct in the development of the mouse enbryo" Mol. Reprod. Dev.36. 279-281 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minami, N.: "Nonspecies-specific effects of mouse oviducts on the development of bovine IVM/IVF embryos by a serum free co-culture" Theriogenology. 41. 1435-1445 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada, M.: "Effects of PHA-P lectin on in vitro development of 1-cell rat embryos." Theriogenology. 43. 359 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim, K. S.: "Effects of bovine follicular fluid on in vitro maturation of bovine oocytes." Jpn. J. Embryo Transfer. 17. 1-5 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosoi, Y.: "Embryo culture in explanted oviducts in mice and cattle." Hormone Res.44. 9-14 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato, H.: "Effects of mouse oviducts on the development of bovine IVM/IVF embryos in a serum free medium." Theriogenology. 41. 224 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi, H.: "Effect of cell surface N-linked carbohydrates on the compaction of preimplantation mouse embryos." J. Soc. Immunol. Reprod.88-92 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hosoi, Y.: "Isolation and characterization of cell lines derived from rat embryos cultured in vitro." Jpn. J. Embryo Transfer. 17. 13-18 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwata, H.: "Effect of taurine on bovine IVM-IVF embryo development co-cultured with camulus cell." Jpn. J. Embryo Transfer. 17. 102-108 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujitani, Y.: "Effects of oxygen tension, free radicals and hypotaurine on development of in vitro-derived bovine embryos." Theriogenology. 45. 210 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim, K. S.: "Functional rale of Cumulus cells during maturation in development of in vitro matured and fertilized bovine oocytes." Theriogenology. 45. 271 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kim, K. S.: "The effects of follicular flued on in vitro maturation, oocyte fertilization and the development fo bovine embryos." Theriogenology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada, M.: "Effects of NaCl and KH_2PO_4 on in vitro development of 1-cell rat embryos in a protein-free defined medium." J.Assist.Reprod.Genet.10. 164 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minami, N.: "Role of the oviduct in the development of the mouse embryo" Mol.Reprod.Dev.36. 279-281 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, H.: "Effects of mouse oviducts on the development of bovine IVM/IVF embryos in a serum free medium" Theriogenology. 41. 224 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minami, N.: "Nonspecies-specific effects of mouse oviducts on the development of bovine IVM/IVF embryos by a serum free co-culture" Theriogenology. 41. 1435-1445 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, M.: "Effects of PHA-P lectin on in vitro development of 1-cell rat embryos." Theriogenology. 43. 359 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi, H.: "Effect of cell surface N-linked carbohydrates on the compaction of preimplantation mouse embryos" J.Soc.Immunol.Reprod.88-92 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosoi, Y.: "Isolation and characterization of cell lines derived from rat embryos cultured in vitro." Jpn.J.Embryo Transfer. 17. 13-18 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim, K.S.: "Effects of bovine follicular fluid on in vitro maturation of bovine oocytes." Jpn.J.Embryo Transfer. 17. 1-5 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hosoi, Y.: "Embryo culture in explanted oviducts in mice and cattle." Hormone Res.44(suppl.2). 9-14 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwata, H.: "Effect of taurine on bovine IVM-IVF embryo development co-cultured with cumulus cell." Jpn.J.Embryo Transfer. 17. 102-108 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujitani, Y.: "Effects of oxygen tension, free radicals and hypotaurine on development of in vitro-derived bovine embryos." Theriogenology. 45. 210 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim, K.S.: "Functional role of cumulus cells during maturation in development of in vitro matured and fertilized bovine oocytes." Theriogenology. 45. 271 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kim, K.S.: "The effects of follicular fluid on in vitro maturation, oocyte fetilization and the development of bovine embryos." Theriogenology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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