1995 Fiscal Year Final Research Report Summary
A new detection method for residual antibiotics using bioluminescent bacteria produced by transformation
| Project/Area Number |
05660367
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Miyazaki University |
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Principal Investigator |
KONDO Fusao Miyazaki University, Faculty of Agriculture, Department of Veterinary Public Health, Professer, 農学部, 教授 (40145404)
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| Project Period (FY) |
1993 – 1995
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| Keywords | Plasmid DNA / Transformation / Microbiological assay / E.coli / Residual antibiotics |
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| Research Abstract |
Using three different plasmid DNAs coding for kanamycin (KM), chloramphenicol (CP) and ampicillin & tetracycline (ABPC & TC) resistance, four different competent Escherichia coli (E.coli) strains were transformed by the calcium chloride method to produce KM-, CP-and ABPC & TC-resistant strains. Evaluation of minimum inhibitory concentration (MIC) of 22 antibiotics, showed KM-resistant E.coli to be cross resistant only to FRM,while CP-resistant E.coli, especially HB101 and JM109 strains, exhibited cross resistance only to thiamphenicol (TP). On the other hand, ABPC & TC resistant E.coli showed cross resistance to several penicillins, tetracyclines and maclorides. E.coli ATCC-27166, the strain most sensitive to all drugs in this experiment, was employed for disc diffusion experiments and from the pattern of appearance of the inhibition zone, eight major antibiotics could be divided into three groups depending on their activity against organisms containing each of the three plasmids. Only gentamicin (GM) was not affected by any of the drug resistant strains. Assay techniques utilizing these resistant strains may allow screening of foods for antibiotic residues in the future.
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