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1994 Fiscal Year Final Research Report Summary

Ultrastructural and immunohistochemical studies on endocytic pathway of high density lipoprotein (HDL) in cells.

Research Project

Project/Area Number 05670034
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNAKAMURA GAKUEN UNIVERSITY,FACULTY OF HOME ECONOMICS

Principal Investigator

FUJITA Mamoru  Nakamura Gakuen University, Faculty of Home Economics Professor, 家政学部, 教授 (60037471)

Co-Investigator(Kenkyū-buntansha) BABA Ryoko  Nakamura Gakuen University, Faculty of Home Economics Associate, 家政学部, 副手
MATSUKUMA Miki  Nakamura Gakuen University, Faculty of Home Economics Associate, 家政学部, 助手 (40259669)
HARA Takayuki  Nakamura Gakuen University, Faculty of Home Economics Assistant professor, 家政学部, 助教授 (10164998)
Project Period (FY) 1993 – 1994
KeywordsHDL / ultrastructure / cytochemistry / histochemistry / LDL / liver / hepatocyte
Research Abstract

We used histochemistry, immunohistochemistry, electromicroscopy, cytochemistry and biochemistry to investigate the endocytic pathway of high density lipoprotein (HDL) in several cells. In addition, we examined the endocytic pathway of low density lipoprotein (LDL) and horseradish peroxidase (HRP) in several cells.
HDL and LDL were isolated from normal rat and rabbit blood by sequential ultracentrifugation. Rat HDL,rat LDL,rabbit HDL and rabbit LDL were labeled with biotin.
The initial interaction of HDL-biotin and LDL-biotin with hepatocytes occurred at binding sites distributed over the basolateral (sinusoidal) plasma membrane. Ligand internalization was involved coated puits, coated vesicles, early endosomes, late endosomes, and lysosomes. However, the binding sites distributed over the endocytic membrane were difference between HDL-biotin and LDL-biotin.
Internalization of HDL-biotin in hepatocytes and adrenal cortical cells of rabbit was no inhibition by a hypercholesterol diet containing 0.5% cholesterol for 4 months.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Mamoru Fujita: "Relationship between lipid transport and cytoshstions in general isorativi cells of the rat" Digestion & Absorption. 17. 64-69 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanenori Hatae: "Occurrence of unusual tubular in vaginations of the plasma membrane in smooth muscle cells of the lamprey,Lampetra-japonica" Cell & Tissue Research. 276. 51-59 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柴田洋三郎: "腸の消化吸収と細胞骨格" 医学のあゆみ. 169. 770-771 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenichirou Morohashi: "Functional difference between Ad4BP and ELP,and their distributions in steroidogenic tissues" Molecular Endocrinology. 8. 643-653 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshiyuki Sakai: "No mutation in cytochrome P450 side chain cleavage in a patient with congenital lipoid adrenal hyperplasia" Journal of Clinical Endocrinology and Metabolism. 79. 1198-1201 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujita, M., Baba, R., Fusano, T., Oshikawa, T.and Miyoshi, M.: "Relationship between lipid transport and cytoskeletons in jejunal absorptive cells of the rat" Digestion & Absorption. 17. 64-69 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatae, T., Ichimura, T., Ishida, T., Sakurai, T.and Fujita, M.: "Occurrence of unusual tubular invaginations of the plasma membrane in smooth muscle cells of the lamprey, Lampetra japonica" Cell Tissue Res. 276. 51-59 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shibata, Y.and Fujita, M.: "Cytoskeletons in intestinal absorption" J.Clinical and Experimental Medicine. 169. 770-774 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morohashi, K., Lida, H., Nomura, M., Hatano, O., Honda, S,Tsukiyama, T., Niwa, O., Hara, T., Takakusu, A., Shibata, .and Omura, T.: "Functional difference between Ad4BP and ELP,and their distributions in steroidogenic tissue" MOL ENDO. 8. 643-653 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakai, Y., Yanase, T., Okabe, Y., Hara, T., Waterman, M.R., Takayanagi, R., Haji, M.and Nawata, H.: "No mutation in cytochrome P450 side chain cleavage in a patient with congenital lipoid adrenal hyperplasia" JCE & M. 79. 1198-1201 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi, M., Fujita, M., Fusano, T., Yano, C., Yamamoto, T.and Kimoto, M.: "Use of soybean peptides to improve bioavailability of methionine and to reduce gastrointestinal soyprotein allergy." REPORT OF THE SOY PROTEIN RESEARCH COMMITTEE (JAPAN). 14. 28-33 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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