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1994 Fiscal Year Final Research Report Summary

Electrophysiological and optical methods for ATP-receptor-operated calcium channels in kidney tubule cells.

Research Project

Project/Area Number 05670038
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionChiba Univ.

Principal Investigator

KAWAHARA Katsumasa  Chiba U.School of Medicrne, Physioly Ass.Pro., 医学部, 助教授 (70134525)

Project Period (FY) 1993 – 1994
Keywordsneonatal rat / Kidney inner medullary collecting duct / P_2 receptor / patch-clamp / confocal laser scanning inicroscope / nuclear envelope / K^+ channel / Ca^<2+> channel
Research Abstract

Neonatal (0-14d) rat inner medullary collecting ducts were isolated by collagenase and were shortly cultured (3-24hr) at 37゚C.Using the patch-clamp technique, I have found two different types of ion channels, Ca-activated potassium channel of 150 pS and non-selective cation channel of 30 pS.Open probabily of both channels was low (<0.001) at the resting condition, but it increased after application of 0.1% serum. These channels may play an important role for cell grawing and regulating ionic contents in the urine. Second, the cells were incubated in a standard solution containing 3muM fluo3-AM for 40 min at 24゚C,their intracellular and nuclear calcium was measured by the confocal laser scanning microscope. The level of fluoresent intensity of nucleoplasm and perinucleoplasm was 19 and 18, respectively. Therefore, a ratio between nuclear and perinuclear intensity was 1.02. Application of ATP (2-100muM) to the bath quickly increased both nuclear and perinuclear intensity with or without calcium in a bathing solution. The N/P ratio also increased to 1.71 (1mM Ca) and 1.53 (Ca free) , suggesting that nuclear calcium increase was synchronized with, but independent from the perinuclear calcium. Although UTP similarly increased the intracellular calcium, AMP and ADP did not. This indicates that a subtype of the P_2-purinergic receptor was P_<2U>.

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Nonaka,T.: "monovalent cation selective channel in the apical membrdre of rat inner medallary collecting duct cells in primary culture." Biochim.Biophys.Acta. 1223. 163-174 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawahara,K.: "Slow activation of chloride currents during hypotonicity in rabbit proximal convoluted tubule(PCT)cells in culture." Jpn.J.Physiol.44:Sup,2. S63-S66 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawahara,K.: "A simple method for continnons measurement of cell herght during a volume change m a single A6 cell." Jpn.J.Physiol.44. 411-419 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawahara,K.: "A stretch-activated cation channel in the apical membrare of A6 cells." Jpn.J.Physiol.43. 817-832 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河原 克雅: "腎尿細管伸展活性化チャネルの生理学" 日本臨床. 51. 2201-2208 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河原 克雅: "パッチクランプ法による腎尿細管イオン輸送研究" 千葉医学. 69. 141-147 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 河原 克雅: "Annual Review腎臓1993" 中外医学社, 249 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawahara, K., Onodera, M., & Fukuda, Y.: "A Simple method for continuous measurement of cell height during a volume change in a single A6 cell." Jpn.J.Physiol. 44. 411-419 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawahara, K.& Matsuzaki, K.: "A Stretch-activated cation channel in the apical membrane of A6 cells." Jpn.J.Physiol.43. 817-832 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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