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1994 Fiscal Year Final Research Report Summary

A GTP-binding peptide regulates beta-adrenergic response of adenylate cyclase

Research Project

Project/Area Number 05670111
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKansai Medical University

Principal Investigator

OMORI Kyoko  Kansai Medical Univ., Dpt.of pharmacol., Lecturer, 医学部, 講師 (90152256)

Project Period (FY) 1993 – 1994
KeywordsGTP-binding / Peptide / beta-adrenergic Response / Adenylate Cyclase / Cytosolic Factor
Research Abstract

We found a cytosolic peptide with a relative molecular weight (Mr)-3,000 in the rat liver which binds to GTP to potentiate the GTP effect on beta-adrenergic response of adenylate cyclase. In the present study, the peptide was purified and characterized. The peptide was isolated as a complex form with GTP on a Sephadex G-25 column in a low salt solution, and was purified as a dissociated form on the column in a high salt solution. The photoaffinity-labeling using [alpha^<-32>P]GTP of the fraction which on the second column showed GTPgammaS-binding activity as well as an enhancing activity of the GTP effect demonstrated that the 3,000 Mr peptide has the GTP-binding property. The fraction was further purified on reverse phase (C_<18>) HPLC.Two peak fractions (fractions I,II) with GTPgammaS-binding activity were obtained, and fraction I eluted with a lower percentage of acetonitrile, enhanced the GTP (10^<-5>) effect maximally 4.8 fold. The amino acid composition analysis of these fractions displayd that fractions I and II are leucine (17 mol/100 mol)- and glycine (34 mol/100 mol)-rich, respectively. To know the primary structure of these peptides, amino acid sequence analysis and the cloning of cDNA are needed.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Toshiko Mikami: "Distribution of neuron-specific clathrin light chain b between clathrin-coated vesicle subclasses." NeuroReport. (Submitted).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tohru Yasukura: "Purification and Characterization of Neurotrophic Factor for Retinal Cholinergic Neurons Derived from Cultured Hippocampal Neurons." Japan.J.Pharmacol.65. 153-162 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kyoko Omori: "A cytosolic peptide potentiates the GTP effect on β-adrenergic response of adenylate cyclase." AM.J.Physiol.266. C498-C507 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiko Mikami et al.: "Distribution of neuron-specific clathrin light chain b between clathrin-coated vesicle subclasses" NeuroReport. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tohru Yasukura et al.: "Purification and Characterization of Neurotrophic Factor for Retinal Cholinergic Neurons Derived from Cultured Hippocampal Neurons." Japan.J.Pharmacol.65. 153-162 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kyoko Omori et al.: "A cytosolic peptide potentiates the GTP effect on beta-adrenergic response of adenylate cyclase." Am.J.Physiol.266. C498-C507 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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