1994 Fiscal Year Final Research Report Summary
Isolation of Epilepsy-Associated Genes from El mice
| Project/Area Number |
05670155
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
MITA Takashi Sch.of Medicine, University of Occupational and Environmental Health Professor, 医学部, 教授 (20076988)
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| Co-Investigator(Kenkyū-buntansha) |
OHMORI Osamu Sch.of Medicine Assistant, 医学部, 助手 (70248558)
FUETA Yukiko Sch.of Medical Tech.Assistant, 助手 (10132482)
HIRANO Hideyasu Sch.of Medicine Assis.Prof., 医学部, 講師 (50040241)
OHMORI Osamu Sch.of Medicine Assistant (70248558)
OHMORI Osamu Sch.of Medicine Assistant (70248558)
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| Project Period (FY) |
1993 – 1994
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| Keywords | epilepsy gene / El mouse / RFLP / cDNA library / synaptosome / Isocitrate dehydrogenase / mutation / S-Adenosylhomocysteine hydrolase |
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| Research Abstract |
Two genes associated with epilepsy of El mice were isolated, one of which encodes S-Adenosylhomocysteine hydrolase (S-AdoHocysHlase). It was derived from cDNA library of liver poly A^+ RNAs of 1 day postnatal El mice. A clone named pELD1 which contains the above gene, revealed Pst I-RFLP on the genomic DNAs between group A (ddY,BALB/c, C3H/He) and group B (El, C57BL/6N,DBA/1J). The latter group includes epileptic seizure predisposed strains. This means that S-AdoHocysHlase gene is associated with the epileptogenesis. Another one was identified by amino acid sequencing to be NADP specific isocitrate dehydrogenase gene, which encodes a 45kDa brain synaptosomal protein appeared in 2D-SDS-PAGE,giving 0.2isoelectric point difference between El and ddY mice. The structure of genes in detail are now under investigation.
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[Publications] Kawano, H., Sashihara, S., Mita, T., Ohno, K., Kawamura, M., Yoshii, K.: "Phenytoin, an antiepileptic drug, competitively blocked non NMDA receptors produced by Xenopus oocytes." Neuroscience Letters. 166. 183-186 (1994)
Description
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