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1994 Fiscal Year Final Research Report Summary

Molecular pathological analysis on the precancerous lesions and early changes in the multistep carcinogenesis of human stomach

Research Project

Project/Area Number 05670173
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionTottori University, Faculty of Medicine

Principal Investigator

ITO Hisao  Department of Pathology, Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (60127610)

Project Period (FY) 1993 – 1994
KeywordsHuman gastric cancer / Precancerous lesion / Molecular pathology / Chromosome aneuploidy / FISH / Apoptosis / Oncogene products / P53 gene
Research Abstract

This study was couducted to examine gastric precancerous lesions molecular pathologically to clarify their significance on the development of gastric cancers.
P53 protein positive cells were noted in intestinal metaplastic mucosa, but not in normal gastric mucasa. PCR-SSCP and fluorescence in situ hydridization (FISH) revealed point mutation of p53 genes (exon 5 and 8) in two of the five specimens with intestinal metaplasia, while loss of the genes was not confirmed. Apoptotic cells were demonstrated by both light microscopy and terminal deoxynucleotidy1 transferase-mediated DUTP-biotin nick end labeling (TUNEL). They distributed in a deeper portion of the metaplastic glands, near proliferative zone, the frequency being higher in incomplete type than in complete type. Metaplastic mucosa expressed c-ERBB2 and c-ERBB3 gene product variably, suggesting the role of their proliferation.
Forty-five gastric tubular adenomas contained apoptotic cells variably, the frequency being significantly (p<0.05) higher in the adenomas showing high grade dysplasia than those with low grade dysplasia. Apoptosis were detected from 7.7 to 14.5% of all gastric cancer cells in 9 well differentiated carcinomas, and from 2.7 to 7.5% in 5 poorly differentiated carcinomas, the frequency being significantly (p<0.01) higher in the former than in the latter. No immunoreactivity for p53 protein was demonstrated in the tubular adenoma, in which no numerical aberration of chromosome 17 and p53 gene was noted. Immunoreactivity for c-ERBB2 and c-ERBB3 gene products was weak in the adenomas.
These results indicated that p53 gene aberration occurred in gastric intestinal metaplasia. Precancerous lesion including metaplastic glands and tubular adenomas variably contained apoptotic cells which play a crucial roles not only in their morphogenesis, but also in the proliferation of the tumor cells.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 安達裕宣: "ヒト胃癌培養細胞株を用いたFISH法の基礎的検討" 消化器癌の発生と進展. 5. 367-370 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N. Kasagi: "Apoptotic cell death in human gastric carcinoma:Analysis by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick endlabeling." Jpn. J. Cancer Res.85. 939-945 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 笠城典子: "Terminai deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL)法によるパラフィン包理切片上でのアポトーシス細胞の可視化:蛋白質解酵素処理の検討を中心として" 米子医学雑誌. 45. 413-423 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Gomyo: "Interphase cytogenetics of gastric carcinoma: Fluorescence in situ hybridization (FISH) applied to cells obtained from formalin-fixed paraffinembedded tissues." Pathology International. 45. 227-232 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] E. Ueno: "Expression of c-ERBB gene family in human gastric mucosa and adenocarcinomas: Analysis by immunohistochemistry and Weastern blotting" Yonago acta medica. 38. 49-56 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito H.: "Springer-Verlag" Gastric Cancer(分担執筆). 446(17) (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ito, H.: "Gastric Cancer" Springer-Verlag. 151-167 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kasagi, N.: "Apoptotic cell death in human gastric carcinoma : Analysis by terminal deoxynucleotidyl transferasemediated dUTP-biotin nick end labeling" Jpn.J.Cancer Res.85. 939-945 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gomyo, Y: "Interphase cytogenetics of gastric carcinoma : Fluorescence in situ hybridizatio (FISH) applied to cells obtained from formalin-fixed paraffin-embedded tissues" Pathology Int. 45. 227-232 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueno, E.: "Expression of c-ERBB gene family in human gastric mucosa and adenocarcinoma : Analysis by immunohistochemistry and Western blotting" Yonago acta medica. 38. 49-56 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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