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1994 Fiscal Year Final Research Report Summary

Pathological significance of homologous restriction factor of complement on the progression of acute myocardial infarction.

Research Project

Project/Area Number 05670178
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionNagoya City University

Principal Investigator

TADA Toyohiro  Nagoya City University Medical School, Department of Pathology, Associate Professor, 医学部, 助教授 (20106230)

Co-Investigator(Kenkyū-buntansha) KUNIMATSU Mitoshi  Nagoya City University Medical School, Depertment of Biochemistry, Assistant Pro, 医学部, 講師 (70145746)
OKADA Hidechika  Nagoya City University Medical School, Institute for Molecular Biology, Professo, 医学部, 教授 (30160683)
Project Period (FY) 1993 – 1994
Keywordscomplement / myocardial infarction / cell death / ischemia / membrane attack complex / inflammation / cardiomyocyte
Research Abstract

Loss of 20 KD homologous restriction factor of complement (HRF20)(CD59) from the cardiomyocytes in the area of myocardial infarction of human hearts was immunohistochemically demonstrated using monoclonal antibody 1F5. Complements deposition including membrane attack complex (MAC) was also immunohistochemically shown on the dead cardiomyocytes in the same (infarcted) area absent from HRF20. In patients of myocardial infarction of 4 hours or less than it of age, neither loss of HRF20 nor complements deposition was found in the myocardium. However, in cases of myocardial infarction of 21 hours or more than it of age, the dead cardiomyocytes in the infarcted area showed MAC deposition and complete loss of HRF20. Decay accelerating factor (DAF)(CD55) was also lost from the infarcted area by the same manner with HRF20. Outside the infarcted area, expression of HRF20 and DAF on the cardiomyocytes outside were well preserved without MAC deposition. Immunoglobulins, especially IgM,were also stained in the dead myocytes in the infarcted area, but no suggestion for its pathological significance and mechanism of deposition were given. Degradation of membrane structure of dead cardiomyocytes would cause removal of HRF20 from infarcted area. The present study suggested that loss of HRF20 from dead cardiomyocytes playd an important role to induce deposition of MAC in the infarcted area.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Toyohiro Tada: "Expression of 20 KD homologous restriction factor of complement on myocardial cells:An immunohistochemical study using acetone-fixed and paraffin-embedded tissues." J.Histochem.Cytochem.41. 119-122 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisashi Tateyama: "Thymic epithelial tumours:evaluation of malignant grade by quantification of proliferating cell nuclear antigen and nucleolar organizer regions." Virchows Archiv A Pathol.Anat.Histopathol.422. 265-269 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chikai Sakurada: "Molecular cloning of the rat complement regulatory protein,5I2 antigen." Biochem.Biophys.Res.Comm.198. 819-826 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsuo Suzuki: "Characterization of protein kinase C activities in post synaptic density fractions prepared from cerebral cortex,hippocampus,and cerebellum." Brain Res.619. 69-75 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitoshi Kunimatsu: "Neutrophil responses induced by formyl and acctyl peptides with the N-terminal sequence of the calpain small subunit." Biochem.Mol.Biol.Int.31. 477-484 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masanori Takeuchi: "Aberrant production of gliostatin/platelet-derived endothelial cell growth factor(PD-ECGF)in rheumatoid synovium." Arthritis Rheum.37. 662-672 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisashi Tateyama: "Proliferative cell nuclear antigen expression in follicular tomours of the thyroid with special reference to oxiphilic cell lesions." Virchows Archiv. 424. 533-537 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuaki Funabashi: "Tissue distribution of complement regulatory membrane proteins in rats." Immunology. 81. 444-451 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsuo Suzuki: "Rapid translation of cytosolic Ca/calmodulin-dependent protein kinase II into postsynaptic density after decapitation." J.Neurochem.63. 1529-1537 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jinnichi Ito: "Rapid and gentle extraction,reconstfuction and characterization of microfilament and glia filament from rat astrocytes." Neurochem.Int.26. 91-97 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwao Ohkubo: "Human seminal plasma b-inhibin:Its purification,characterization,and Immunohistochemical localization." Int.J.Biochem.(発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyohiro Tada, Hidechika Okada, Noriko Okada, Hisashi Tateyama, Tadaaki, Eimoto: "Expression of 20 KD homologous restriction factor of complement on myocardial cells : An immunohistochemical study using acetone-fixed and paraffin-embedded tissues." J.Histochem.Cytochem. 41. 119-122 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisashi Tateyama, Tsutomu Mizuno, Toyohiro Tada, Tadaaki Eimoto, Takahiko Hashimoto, Akira Masaoka : Thymic Epithelial tumours: "Evaluation of malignant grade by quantification of and nucleolar organizer regions." Virchows Archiv Pathol.Anat.422. 265-269 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chikai Sakurada, Hachiro Seno, Natsuki Dohi, Hisao Takizawa, Masaru Nonaka, Noriko Okada, Hidechika Okada: "Molecular cloning of the rat complement regulatory protein, 5I2 antigen." Biochem.Biophys.Res.Comm.198. 819-826 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsuo Suzuki, Kuniko Okumura-Noji, Ryo Tanaka, Akihiko Ogura, Kyoko Nakamura, Yoshihisa Kudo, Toyohiro Tada: "Characterization of protein kinase C activities in post synaptic density fractions prepared from cerebral cortex, hypocampus, and cerebellum." Brain Res.619. 69-75 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitoshi Kunimatsu, Xiao Jing Ma, Sasuhiko Ozaki, Joh Nishimura, Shunkichi Baba, Makoto Sasaki: "Neutrophil responses induced by formyl and acetyl peptides with the N-terminal sequences of the calpain small subunit." Biochem.Mol.Biol.Int.31. 477-484 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masanori Takeuchi, Takanobu Ohtsuka, Nobuo Matsui, Kiyohumi Asai, Takayoshi Hirano, Akihiko Moriyama, Ichiro Isobe, Yaman Z.Eksioglu, Kohei Matsukawa, Taiji Kato, Toyohiro Tada: "Aberrant production of gliostatin/platlet-derived endothelial cell growth factor in rheumatoid synovium." Arthritis Rheum.37. 662-672 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisashi Tateyama, Yi-Ping Yang, Tadaaki Eimoto, Toyohiro Tada, Hiroshi Inagaki, Takaaki Nakamura, Hirotaka Iwase, Syunzou Kobayashi: "Proliferative cell nuclear antigen expression in follicular tomours of the thyroid with special reference to oxiphilic cell lisions." Virchow Archiv. 424. 533-537 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Funabashi, N.Okada, S.Matsuo, T.Yamamoto, B.P.Morgan, H.Okada: "Tissue distribution of complement regulatory membrane proteins in rats." Immunology. 81. 444-451 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsuo Suzuki, Kuniko Okumura-Noji, Ryo Tanaka, Toyohiro Tada: "Rapid translocation of cytosolic Ca/calmodulin-dependent protein kinase II into postsynaptic density after decapitation." J.Neurochem.63. 1529-1537 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jinnichi Ito, Mari Masuda, Toyohiro Tada, Ryo Tanaka: "Rapid and gentle extraction, reconstruction and glia filament from rat astrocytes." Neurochem.Int.26. 91-97 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwao Ohkubo, Toyohiro Tada, Yukiko Ochai, Hisao Ueyama, Tadaaki Eimoto, Makoto Sasaki: "Human seminal plasma b-inhibin : Its purification, characterization, and Immunohistochemical localization." Int.J.Biochem.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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