1994 Fiscal Year Final Research Report Summary
Application of multi-purpose tissue fixation method (AMEX method) to the clinical laboratory
| Project/Area Number |
05670181
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Kitasato University |
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Principal Investigator |
SATO Yuichi Kitasato University School of Medicine, lecturer, 医学部, 講師 (30178793)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Tissue / paraffin-embedding method / DNA / protein / 電気泳動 |
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| Research Abstract |
We developed a new fixation and paraffin-embedding method of tissues (AMeX fixation method) that preserves many antigens as well as high-molecular weght DNA,RNA and protein that are destroyed by routine formalin fixation and paraffin-embedding method. Tissue constructions also preserved.
In this study, we examined various kinds of requirements to apply to the pathology laboratory for AMeX fixation method with automatic tissue processor. As a result, we established a following procedure. At first, tissues were fixed by cold acetone at least one day to one week. Then, tissues were dehydrated and penetrated by automatic tissue processor (SAKURA's Tissue Rotary) , in which tissues were immersed for 30 min each in 4 acetone, 2 methyl benzoate, 2 xylene, 4 paraffin, respectively. Finally, tissues were embedded in paraffin and stored at 4 C.
By this way, we could apply the AMeX fixation method to the clinical laboratory as a routine work. Differences for the preservation of morphology and antigen as well as DNA and protein between tissues fixed by automatic AMeX fixation method and original AMeX method were not observed. Tissues fixing in both method was able to detect various kinds of antigens which could not detect routinely formalin-fixed and paraffin-embedding tissues. It will be able to carry out the AMeX fixation method by this means in many institutions.
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