A role of T cell receptor beta on immature thymoctye differentiation

1994 Fiscal Year Final Research Report Summary

• Project/Area Number: 05670304
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY (1994); Kyushu University (1993)
• Principal Investigator: KISHI Hiroyuki Toyama Med.Pharm.Univ., Facult.Med.Assistant professor, 医学部, 助教授 (60186210)
• Project Period (FY): 1993 – 1994
• Keywords: Thymus / Cell surface antigen / Differentiation

Research Abstract

A monoclonal antibody, 1-23, which recognizes a novel cell surface antigen on immature murine thymocytes (designated as IMT-1 for immature thymocyte antigen-1) was prepared.IMT-1 was found to be expressed on 40-50% of CD4^-8^- (DN), 5-10% of CD4^-8^+ and 5-20% of CD4^++^8 (DP) thymocytes in adult mice, but not expressed on CD4^+8^- thymocytes or peripheral T lymphocytes. In the subset of DN thymocytes, IMT-1 was detected on 40 to 50% of the heat stable antigen^+ (HSA^+), 15% of CD44^+25^+,70% of CD44^-25^+ and 70% of CD44^-25^- populations, whereas it was not detected on HSA^- and CD44^+25^- populations. In fetal thymuses, IMT-1 was expressed on a minor population of thymocytes at day 14.5 and 15.5 of gestation. However, at day 16.5 of gestation, a majority of DN as well as DP thymocytes became IMT-1 positive. These results shows that the expression of IMT-1 is correlative to that of pre-TCR.Addition of the 1-23 antibody to the long-term fetal thymus organ culture reduced the DP thymocyte population. These results indicate the possibility that the IMT-1 may be involved in the regulation of thymocyte differentiation.

Research Products

• [Publications] Kishi,H.: "Cell surface antigen,IMT-1,expressed on thymocytes differentiating from CD4^-8^- to CD4^+8^+" J.Cell.Biochem.180. 455- (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tagoh,H.: "Reciprocal IL-1 β gene expression in medial and lateral hypothalamic areas in the SART-stressed mice." Nurosci.Lett.184. 17-20 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kishi, H., Su, D.M.and Watanabe, T.: "Cell surface antigen, IMT-1, expressed on thymocytes differentiating from CD4-8-to CD4_+8_+." J.Cell.Biochem.18D. 455 (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tagoh, H., Nishijo, H., Uwano, T., Kishi, H., Ono, T., and Muraguchi, A.: "Reciprocal lL-1beta gene expression in medial and lateral hypothalamic areas in the SART-stressed mice." Neurosci.Lett.184. 17-20 (1995)
  • Description: 「研究成果報告書概要(欧文)」より