1994 Fiscal Year Final Research Report Summary
Cellular communication of the liver using molecular techniques
| Project/Area Number |
05670452
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | University of Tokyo |
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Principal Investigator |
SHIRATORI Yasushi University of Tokyo, Dept.Int.Med., Assistant, 医学部(病), 助手 (70196624)
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| Co-Investigator(Kenkyū-buntansha) |
KOMATSU Yukata University of Tokyo, Dept.Int.Med., 医学部(病), 医員
MATSUMURA Masayuki University of Tokyo, Dept.Int.Med., 医学部(病), 医員
NIWA Yasuro University of Tokyo, Dept.Int.Med., 医学部(病), 医員
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| Project Period (FY) |
1993 – 1994
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| Keywords | hepatic sinusoid / endothelial cells / Kupffer cells / liver-associated NK cells / TGF-alpha / CINK / IL-2 / molecular technique |
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| Research Abstract |
Liver lobule consists of parenchymal cells and four types of sinusoidal cells. Establishment and maintenance of hepatic sinusoidal endothelial cells have been investigated. Kupffer cells release several types of growth factors for hepatic endothelial cells in vitro, and some of them are TGF-alpha. During proliferation of the sisnuoidal endothelail cells, PKC expression increased in the cells. In addition, extracellular matrix modulated morphological changes of hepatic endothelial cells during angiogenesis.
Liver-associated NK cells play an important role in immune defense system in the liver, which has been found to be increased by interleukin 2 produced by macrophages upon stimulation of biologically responsive mediators. tn enhanced transcription level of IL-2 in macrophages has been demonstrated by PCR.
In addition, infiltrating of neutrophils has been analyzed by measuring the transcription level or protein production of interleukin-8, cytokine-induced neutrophil chemoattractant (CINK) in rodent. Production of this mediator has been also modulated by cytokine network in the liver as well.
Present study clearly demonstrated cellular communication in the liver by molecular techniques.
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[Publications] Shiratori Y,Takada H,Hikiba Y,Nakata R,Okano K,Komatsu Y,Niwa Y,Matsumura M,Shiina S,Omata M,Kamii K: "Production of chemotactic factor, in terleukin 8, from hepatocytes exposed to ethanol" Hepatology. 18. 1477-1487 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Komatsu Y,Shiratori Y,Kawase T,Hashimoto N,Han kk, Shiina S,Matsumura M,Niwa Y,Kato N,Tada M,Ikeda Y,Tanaka M,Omata M: "Role of polymorphonuclear leukocytes in galactosamine hepatitis." Hepatology. 20. 1548-1556 (1994)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shiratori Y,Hikiba Y,Mawet E,Niwa Y,Matsumura M,Kato N,Shiina S,Tada M,Komatsu Y,Kawabe T,Yoshida H,Okano K,Omata M: "Modulation of KC/Gro Protein (interleukin-8 related protein in rodent) release from hepatocytes by biologically active mediators." Biochem Biophys Res Commun. 203. 1398-1403 (1994)
Description
「研究成果報告書概要(欧文)」より
