1995 Fiscal Year Final Research Report Summary
Studies on pathogenesis and progression of liver cirrhosis -with special reference to the hepatic sinusoidal actomyosin sygtems
| Project/Area Number |
05670495
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Department of Internal Medicine, School of Medicine, Keio University |
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Principal Investigator |
ODA Masaya Sch.of Med.Keio Univ.Dept.of Int.Med.Assist.Prof., 医学部, 専任講師 (20129381)
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| Co-Investigator(Kenkyū-buntansha) |
FUNATSU Kazuo Sch.of Med.Keio Univ.Dept.of Int.Med.Assist.Prof., 歯学部, 助教授 (00129644)
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| Project Period (FY) |
1993 – 1995
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| Keywords | liver cirrhosis / hepatic sinusoidal endothelial cells / hepatic stellate cells (Ito cells) / Kupffer cells / Ca^<++> -calmodulin-actomyosin system / electron microscopy / Fura-2AM / FITC labelled endotoxin |
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| Research Abstract |
By transmission electron microscopy and immunohistochemistry, actin and myosin filaments and Ca^<++>-calmodulin actomyosin system are evident in the hepatic sinusoidal endothelial cells, hepatic stellate cells (Ito cells) and Kupffer cells. Immunohistochemistry and the dynamic analysis of intracytoplasmic free Ca^<++> ion using Fura2-AM showed that the Ca^<++> -calmodulin actomyosin system regulates the contraction and dilatation of the sinusoial endothelial fenestrae (SEF), contaction of Ito cells and phagocytotic activities of Kupffer cells.
Scanning and transmission electron microscopy revealed that the diameter and numbers of the SEF were significantly decreased concomitant with the fibrosis within the space of Disse in cirrhotic liver.
By quantitative analysis, the phagocytotic activity of Kupffer cells against the FITC-labelled endotoxin have been shown to be significantly decreased in cirrhotic liver. Furthermore, electron microscopic observations have proved that the microparticles injected via a mesenteric vein catheter are decreased in space of Disse in cirrhotic liver as compared with control liver.
These finding may indicate that the enhancement of a decrease of hepatic sinusoidal blood flow results from a decrease of SEF in diameter and number and a decrease of phagocytotic activity of Kupffer cells, both of which have some relationship with an increase of serum endotoxin levels in cirrhotic liver.
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[Publications] Oda, M., Kaneko, H., Suematsu, M., Suzuki, H.Kazemoto, S., Honda K., Yonei, Y.and Tsuchiya, M.: "A new aspect of the hepatic microvasculature : electron microscipic evidence for the presence of Ito cells around portal and hepatic venules as pericytes" Prog.Appl.Microcirc.19. 25-39 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Oda, M., Kazemoto, S., Kaneko, H., Yokomori, H., Ishii, K., Tsukada, N., Watanabe, N., Suematsu, M.and Tsuchiya, M.: "Involvement of Ca^<++>-calmodulin-actomyosin system in contractility of hepatic sinusoidal endothelial fenestrae." Cells of the Hepatic Sinusoid. 4. 174-178 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Takaishi, H., Oda, M., Yokomori, H,Kaneko, H., Kazemoto, S., Suzuki, H., Suematsu, M., Komatsu, H.and Tsuchiya, M.: "A role of Ito cells in the hepatic microcirculation -Evidence for their contractility and actin filaments in primary culture-" Microcirculation annual. 9. 99-100 (1993)
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[Publications] Kazemoto, S., Oda, M., Kamegaya, Y., Kaneko, H., Kaneko, K., Akiba, Y., Nakamura, M.and Tsuchiya, M.: "Involvement of capsaicin-sensitive neurons in the peptidergic nervous control of hepatic microcirculation in the rat." Microcirculation annual. 10. 107-108 (1994)
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[Publications] Funatsu, K., Matsumaru, A., Itsuji, S., Ueno, H., Arakawa, S., Takagi, T., Ebihara, Y., Mizuno, Y., Oda, M.and Ishii, H.: "Hepatocellular injury due to active oxygen radicals produced by rat liver macrophages in vivo and in vitro." Cells of the Hepatic Sinusoid. 5. 48-50 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Kamegaya, Y., Oda, M., Kazemoto, S., Yokomori, H., Kaneko, H., Honda, K., Ishii, H.and Tsuchiya, M.: "Evidence for the spontanous contractility of Ito cells. -Time-lapse cinematography and computarized image analysis." Cells of the Hepatic Sinusoid. 5. 306-307 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Oda, M., Kamegaya, Y., Akiba, Y., Yokomori, H.and Nakamura, M.: "Peptidcrgic nervous control of hepatic microcirculation -a review and new aspect, In : Llver Innervation. Edited by T.Shimazu." John Libbey and Company, London, Paris, Rome. 255-264 (1996)
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[Publications] Kamegaya, Y., Oda.M., Yokomori, H., Han, J.-Y., Akiba, Y., Nakamura, M.and Ishii, H.: "Prostaglandin El enhances hepatic microcirculation. A role of Ca^<++>- ATPase. Microcirculation, Edited by K.Messmer and W.M.Kbler." Monduzzi Editore, Bologna.939-942 (1996)
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