1994 Fiscal Year Final Research Report Summary
Role of Scavenger Receptor in Human Alveolar Macrophages
| Project/Area Number |
05670530
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
SUGA Moritaka 1ST DEPARTMENT OF INTERNAL MEDICINE,KUMAMOTO UNIVERSITY SCHOOL OF MEDICINE ; INSTRUCTOR, 医学部, 講師 (20154437)
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| Project Period (FY) |
1993 – 1994
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| Keywords | alveolar macrophage / scavenger receptor / in situ hybridization / clearance of forein substances / pulmonary alveolar proteinosis / idiopathic pulmonary fibrosis / pneumoconiosis / sarcoidosis |
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| Research Abstract |
To clarify the role of scavenger receptor (SR) in human alveolar macrophages (AM), the expressions of SR in AM obtained from normal healthy volunteers (NHY) and patients with pulmonary diseases ; pulmonary carcoidosis (Sar), slicosis (Silic), idiopathic pulmonary fibrosis (IPF), and pulmonary alveolar proteinosis (PAP) by immunohistochemical and immunoelectron microscopical technique using an antibody aginst human macrophage scavenger receptors, hSR I-2, and by in situ hybridization using human scavenger receptor cDNA,were examined.
The expression of SR among AM from patients with Sar (92 <plus-minus> 4.6%), with Silic (87 <plus-minus> 4.9%), and with IPF (87 <plus-minus> 5.5%) did not sugnificantly decrease compared with those of NHV (91.2 <plus-minus> 4.8%). However, in AM from patients with PAP the expression was significantly suppressed (64.0 <plus-minus> 7.5%). It was confirmed that the expression of SR mRNA by in situ hybridization decreased in AM from patients with PAP.These results showed that in AM from patients with PAP the expression of SR is suppressed, and SR in human AM may play important role on clearance of some components of pulmonary s
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