1995 Fiscal Year Final Research Report Summary
Biological activity of the ANAE-1 derived from the granuloma macrophage
| Project/Area Number |
05670531
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Oita Medical University |
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Principal Investigator |
TSUDA Tomiyasu Oita Medical University Faculty of Medicine, professor, 医学部, 教授 (80040567)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Eishi Oita Medical University Faculty of Medicine, Research Associate, 医学部, 助手 (00264333)
YUBISUI Toshitsugu Kochi Uiversity, professor, 理学部, 教授 (00019564)
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| Project Period (FY) |
1993 – 1994
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| Keywords | ANAE-1 / MIF / epitherioid cell granuloma |
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| Research Abstract |
We purified the ANAE-1 from adjuvant-induced rabbit lung granuloma for analyzing the biological function of it. We homogenized granulomatous tissue resected from the lungs of rabbit that had received an Freund's complete adjuvant injection 4 weeks before. The ANAE-1 was purified as a single band by SDS-PAGE with standard method of chlumn chromatography. The purified ANAE-1 have showed macrophage migration inhibition activity over 80 nM.We tried to analyze the amino acid sequence of the ANAE-1 for molecular analysis. But we do not success because the ANAE-1 is not a single protein by isoelectric focusing.
On the other hand, when we examined whether human epithelioid cells have a specific ANAE-isozyme as well as rabbit epithelioid cells have the ANAE-1, any specific isozymes were observed in human epithelioid cells compared with human alveolar macrophages.
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