1994 Fiscal Year Final Research Report Summary
Effect of insulin like-growth factor on development and survival of cholinergic neurons
| Project/Area Number |
05670582
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
TAKAHASHI Keikichi National Center of Neurology and Psychiatry National Institute of Neuroscience Section Chief, 神経研究所, 室長 (40117148)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Cholinergic neuron / Neurotrophic factor / choline acetyltransferase / Interleukin-3 / Insulin-like growth factor / Insulin-like growth factor II receptor |
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| Research Abstract |
We investigated the effect of insulin-like growth factors II (IGFII) on septal primary cultures from mouse embryonic day 15 brains. The addition of IGFII to septal cultures enhanced total choline acetyltaransferase (ChAT) activity in a dose-dependent manner. The effect of IGFII on ChAT activity was completely blocked by anti-IGFII/M-6-P receptor antibodies, whereas the antisera alone had no effect on the enzyme activity. Double-labeled immunohistochemical studies revealed that most ChAT-positive neurons expressed IGFII/M-6-P receptor immunoreactivity. These results indicate that the trophic effect of IGFII results from the direct action of this molecule through the IGFII/M-6-P receptor in septal cholinergic neurons. We also examined expression and production of interleukin-3 (IL-3) mRNA and IL-3protein in mouse primary cultured neurons and glia by the reverse transcription and polymerase chain reaction and bioassay using an IL-3-dependent cell line. IL-3 mRNA was demonstrated mainly in hippocampal neurons but not in glia, while a small but definite production of bioactive IL-3 was detected in septal and hippocampal neuronal cultures. Thus, endogenous IL-3 might be produced by certain neurons in situ.
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