1994 Fiscal Year Final Research Report Summary
Morphological and functional studies on blood cells in hematological diseases of patients with abnormal chromosomes.
| Project/Area Number |
05670694
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
EGUCHI Mitsuoki Dokkyo University, Dept.Medicine, Associate Professor, 医学部, 助教授 (60020799)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Down's syndrome / Trisomy 13 / Trisomy 22 / Fanconi's syndrome / MDS (myelodys plastic syndrome / a cute megakaryoblastic leukemia / peroxidase / DNA |
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| Research Abstract |
I observed blood cells in hematological diseaes of patients with abnormal chromosomes by using electron microscopical and ultracytochemical techniques, and compared with those in non-hematological conditions and in normal chromosomes.
1. Down's syndrome : (1) Electron microscopically, blood cells were normal in the patients without hematological disease. (2) The blast cells from patients with transient abnormal myelopoiesis (TAM) had characteristics of neutrophils, basophils and erythroblasts, other than megakaryocytes. (3) When they were transformed to megakaryoblastic leukemia, the blasts showed less-differenciated form without alpha graunles, and reduced multipotential features.
2. Trisomy 22 : Patients without hematological diseases showed fewer peroxidase-positive granules in neutrophils than those in normal controls.
3. Trisomy 13 : The numbers of peroxidase-positive granules were more than those in normal controls.
4. Multiple trisomy : Neutrophils from a patient with multiple trisomy had nuclear pockets and Auer bodies.
5. Fanconi's syndrome : Pateints with only anemia had no fine structural abnormality in blood cells. The patient with MDS revealed deformed nuclei in blood cells. However, when he transformed to MDS,blasts showed no more deformed nuclei.
In conclusion, the blood cells from patients with abnormal chromosomes showed finestructural abnormality though they were normal in light microscopy. The blood cells in those patients with hematological diseases were more abnormal in structure and cytochemistry than those from patients with normal chromosomes.
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