The regulation of gene expression for the genes associated with eosinophils.

1994 Fiscal Year Final Research Report Summary

• Project/Area Number: 05670927
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Jichi Medical School
• Principal Investigator: YAMAGUCHI Yuji Jichi Medical School, Lecturer, 医学部, 講師 (00220286)
• Co-Investigator(Kenkyū-buntansha): KASAHARA Tadashi Jichi Medical School, Lecturer, 医学部, 助教授 (60049096)
• Project Period (FY): 1993 – 1994
• Keywords: GATA-1 / eosinophil / Eosinophil peroxidase / Charcot-Leyden crystal / Transcription factor

Research Abstract

The analysis of the regulation of gene expression for the genes associated with eosinophils were caried out.
1.GATA-1 plays an important role in the eosinophil defferentiation of eosinophilic cell line, HL-60-C15 cells, and normal human bone marron cells.
2.In the promoter for the gene encoding human eosinophil peroxidase, a number of positively and negatively acting elements were identified.
3.The regulatory regions of Charcot-Leyden crystal protein were identified.

Research Products

• [Publications] Yuji Yamaguchi: "Functional Characterization of the Promoter for the Gene Encoding Human Eosinophil Peroxidase" J.Biol Chem.269. 19410-19419 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Gomolin, H.I., Yamaguchi, Y., Palupillai, A.V., Dvorak, L.A., Ackerman, S.J., Tenen, D.G.: "Human eosinophil Charcot-Leyden crystal protein : Cloning and characterization of a lyso phospholipase gene promoter." Blood. 82. 1868-1874 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Zon, L.I., Yamaguchi, Y., Yee, K., Albee, E.A., Kimura, A., Bennett, J.C., Orkin, S.H., Ackerman, S.J.: "Expresion of mRNA for the GATA-binding proteins in human eosinophils and basophils : Potential role in gene transcription." Blood. 81. 3234-3241 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamaguchi, Y., Zhang, D.E., Sun, Z., Albee, E.A., Nagata, S., Tenen, D.G., Ackerman, S.J.: "Functional characterization of the promoter for the gene encoding human eosinophil peroxidase." J.Biol.Chem.269. 19410-19419 (1994)
  • Description: 「研究成果報告書概要(欧文)」より