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1994 Fiscal Year Final Research Report Summary

Primary culture of proximal tubular cells (PTC) from normal mouse kidney as an in vitro model to study mechanisms of development of tubulointerstitial nephritis

Research Project

Project/Area Number 05670944
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Kidney internal medicine
Research InstitutionCHIBA UNIVERSITY

Principal Investigator

UEDA Shiro  CHIBA UNIVERSITY,SCHOOL OF MEDICINE,LECTURER, 医学部, 講師 (50201348)

Co-Investigator(Kenkyū-buntansha) HORI Junro  CHIBA UNIVERSITY,SCHOOL OF MEDICIN,FELLOW, 医学部・附属病院, 医員
OGAWA Makoto  CHIBA UNIVERSITY,CHIBA UNIVERSITY HOSPITAL,ASSISTANT, 医学部・附属病院, 助手 (50241956)
Project Period (FY) 1993 – 1994
KeywordsTubular basement membrane (TBM) anrigen / Cell culture / Proximal tubular cells (PTC) / Tubulointerstitial nephritis / Intercellular adhesion molecule-1 (ICAM-1)
Research Abstract

Cultured murine PTC clearly expressed ICAM-1 under the influence of TBM-primed T lymphocytes. These lymphocytes also demonstrated a high cytotoxic activity against PTC.Purified PTC from BALB/c mice was primary-cultured. PTC was prepared by a modified method of Bogaard et al (Biochem Pharmacol 39 : 13351345,1990). Briefly, kidney was perfused with buffer containing 0.08% (w/v) collagenase. Then, the cortical tissue was filtered through a nylon-gauze. Viable PTC was separated from other materials by isopycnic centrifugation on a discontinuous Nycodenz gradient. The confluent monolayr of PTC showed typical epithelial morphology with cobblestone-like appearanc. Typical dome formation was observed in PTC cultures. These cells also expressed gamma-GTP activity. TBM-primed nylon-column non-adherent lymphocytes were prepared from BALB/c mice immunized with mouse TBM Ag in adjuvant.
Cocultured PTC with TBM-primed lymphocytes induced ICAM-1 expression in PTC.The lymphocytes also had a strong cytotoxic activity without complement. Depletion of T cells from these lymphocytes removed the cytotoxic activity. Neither liver antigen-primed nor DNP-primed lymphocytes had any cytotoxicity against PTC.
This simple syngeneic model may allow further molecularbiological examination of tubulointerstitial nephritis.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 堀潤朗他8名: "培養尿細管上皮細胞を用いての間質性腎炎発症機序の解析-尿細管基底膜(TBM)抗原感作リンパ球にBICAM-1の誘導" 日本腎臓学会試. 37(3月25日発行予定). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上田志朗: "Annual Review腎臓" 中外医学社, 5 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiro Ueda: "Clinical Nephrotoxins" Decker,New York, 15 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hori J,Ueda S,Anzai N,Itoh K,Ogawa M,Ohto M,Wakashin Y,Wakashin M: "Primary culture of proximal tubular cells(PTC)from normal mouse kidney as an in vitro model to study mechanisms of development of tubulointerstitial nephritis-Induction of ICAM-1 in PTC by antigen-primed lymphocytes." Jap J Nephrol. 37 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiro Ueda: Nephrotoxicity of D-penicillamine, gold salts, and alloprinol.in "Clinical Nephrotoxins" , edt by Bennett WN,De Broe ME,Porter GA,and Varpooten GA.Decker, New York, (in press), (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiro Ueda: Gold nephropathy, in "Annual Review Jinzo 1994" , edt by Kosikawa S,Nagasawa T,Koiso K,Itoh T.CHUGAI IGAKU Co.Tokyo, 102-106 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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