1994 Fiscal Year Final Research Report Summary
Quantitative assay of cytokine mRNA expression in peripheral blood mononuclear cells and its clinical application
| Project/Area Number |
05671012
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
ARINAGA Shinya KYUSHU UNIVERSITY MEDICAL INSTITUTE OF BIOREGULATION,ASSISTANT PROFESSOR, 生体防御医学研究所, 講師 (70151181)
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Hideaki KYUSHU UNIVERSITY MEDICAL INSTITUTE OF BIOREGULATION,LECTURER, 生体防御医学研究所, 助手 (20253528)
INOUE Hiroshi KYUSHU UNIVERSITY MEDICAL INSTITUTE OF BIOREGULATION,LECTURER, 生体防御医学研究所, 助手 (90203249)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Cytokine / Messenger RNA / Quantitative assay / Interleukin l-alpha / Interleukin l-beta / Interleukin 2 / Tumor necrosis factor-alpha / Immunochemotherapy |
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| Research Abstract |
A quantitative assay for the expression of cytokine mRNA in peripheral blood mononuclear cells (PBM) has been developed using a quantitative RT-PCR method, in which cRNA of each cytokine is employed as inner control. Utilizing this assay system, mRNA expression of IL1-alpha、IL1-beta or TNF-alpha was quantitatively assessed in PBM after activation with IL2 in vitro. Both IL1-alpha and -beta mRNA expression was biphasically increased by 1 to 3 hr and 6 to 12 hr after activation, and thereafter decreased. However, expression of TNF-alpha mRNA was gradually increased after 24 to 48 hr stimulation following the peak level of evelation by 1 to 3 hr. Further, these cytokine mRNA expressions in PBM were evaluated in patients with carcinoma following the treatment with IL2 in vivo. The increased expression of IL1-alpha or -beta mRNA was observed 1 day after the treatment, while TNF-alpha mRNA was differentially expressed following the therapy.
These results indicate that this assay system could be useful for the investigation of the influence of various BRM on cytokine-network in cancer patients and, therefore, for the evaluation of the effectiveness of these BRM on cancer.
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