1994 Fiscal Year Final Research Report Summary
Long-term effects of small bowel transplantation on intestinal motility.
| Project/Area Number |
05671035
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
KUSUNOKI Masato Hyogo College of Medicine Research Associate, 医学部, 助手 (50192026)
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| Project Period (FY) |
1993 – 1994
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| Keywords | NANC |
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| Research Abstract |
We previously found that the contractile motility of the jejunum was increased 4 weeks after transplantation, and that dominant intrinsic neural component was changed from cholinergic to non-adrenergic, non-cholinergic (NANC). The present study investigated the long-term effects of transplantation on jejunal motility using rats that survived for 2 years postoperatively. Jejunal strips were harvested various group of rats, and intestinal motility was assessed by electrical transmural stimulation. Stimulation produced a similar increase of contraction at 4 weeks and 2 years after grafting. Pretreatment with atropine showed that the cholinergic component of contraction was respectively 45%, 24%, 32%, and 24% in adult controls, adult rats 4 weeks after transplantation, 2-year-old controls, and rats 2 years after transplantation. The NANC component (obtained with atropine and guanethidine) in each group was respectively 56%, 73%, 60%, and 69%. The actual valve of tetrodotoxin-insensitive myogenic component was significantly increased at 2 years after transplantation. These results suggested that the NANC component has a key role in the motility of transplanted small bowel throughout the life of the grafts.
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