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1994 Fiscal Year Final Research Report Summary

Microspectrophotometric analysis of DNA content in duct epithelial proliferation and invasive carcinoma of the panece

Research Project

Project/Area Number 05671047
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionThe University of Tokyo

Principal Investigator

NAGASHIMA Ikuo  Univ.Tokyo, Assistant, 医学部・附属病院, 助手 (90202423)

Co-Investigator(Kenkyū-buntansha) YAMAGATA Seiichi  Univ.Tokyo, Assistant, 医学部・附属病院, 助手
KIMURA Wataru  Univ.Tokyo, Assistant, 医学部・附属病院, 助手 (00169947)
WADA Yoshiyuki  Univ.Tokyo, Assistant, 医学部・附属病院, 助手 (70107647)
KURODA Akira  Univ.Tokyo, Director, 医学部・附属病院, 講師 (70010270)
Project Period (FY) 1993 – 1994
Keywordspancreatic duct proliferation / pancreatic cancer / DNA ploidy pattern / microspectrophotometry / precarcinoma
Research Abstract

Nuclear DNA content of 131pancreatic duct epithelial lesions, including 10 normal ducts, 30 intraductal proliferations with mibel atypia (group I-II), 30 with moderate atypia (group III), 24 with severe atypia (group IV), 14 of carcinoma in situ (groupV), and 23 invasive carcinomas, was analyzed using microspectrophoto metry. DNA histograms. were cl asified into diploid, polyploid and aneuploid patterns.
All of normal duct epithelia showed diploidy. Polyploid patterns a observed in 3 (10%) lesions of groups I-II,17 (56.7%) of group III 14 (58.4%) of group IV,7 (50%) of group V,and 6 (26.1%) of in vasive carcinomas, and aneuploid patterns were observed in 0%, 10%, 33.3 50% and 73.9%, respectively. This distribution of ploidy patterns re vealed a gradual shift to the main ploidy from diploid to polyploid followed by aneuploid in proportion to the increase of the degree of epithelial atypia.
The frequencies of polyploid cells in each lesiong were determined. Their averages were 0.2% in group I-II,1.9% in group III,3.4% in group IV,4.4% group V,and 6.7% in invasive carcinoma. The S・G_2M phase fractions were Significantly higher in proliferative epithelia than in normal. The results of the study suggest that duct epithelial proliferations of the pancreas have "go instability" leading to a serial clonel evolution and play a significant role in the progressions of pancreatic duct cell carcinoma.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 金政錫・他: "顕微蛍光測光法による核DNA分析からみた膵癌・乳頭部癌の組織発生" 胆と膵(特集). 13(3). 283-290 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金政錫・他: "顕微蛍光測光法による膵管上皮増生巣の核DNA量解析" 日本消化器病学会雑誌. 89(5). 1286-1296 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Kin et al.: "Development of parcreas ad papilla vatenx cortinoma from DNA analysis with fluorecein microscopes" Gall lladde ad Parcres. B. 283-290 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S Kin et al.: "DNA analysis of prolifurative pancreatic duct epithelium with fluoresein microscopes" Jap. J.Bastroentenlogy. 89(5). 1281-1296 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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