1994 Fiscal Year Final Research Report Summary
Microspectrophotometric analysis of DNA content in duct epithelial proliferation and invasive carcinoma of the panece
| Project/Area Number |
05671047
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAGASHIMA Ikuo Univ.Tokyo, Assistant, 医学部・附属病院, 助手 (90202423)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGATA Seiichi Univ.Tokyo, Assistant, 医学部・附属病院, 助手
KIMURA Wataru Univ.Tokyo, Assistant, 医学部・附属病院, 助手 (00169947)
WADA Yoshiyuki Univ.Tokyo, Assistant, 医学部・附属病院, 助手 (70107647)
KURODA Akira Univ.Tokyo, Director, 医学部・附属病院, 講師 (70010270)
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| Project Period (FY) |
1993 – 1994
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| Keywords | pancreatic duct proliferation / pancreatic cancer / DNA ploidy pattern / microspectrophotometry / precarcinoma |
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| Research Abstract |
Nuclear DNA content of 131pancreatic duct epithelial lesions, including 10 normal ducts, 30 intraductal proliferations with mibel atypia (group I-II), 30 with moderate atypia (group III), 24 with severe atypia (group IV), 14 of carcinoma in situ (groupV), and 23 invasive carcinomas, was analyzed using microspectrophoto metry. DNA histograms. were cl asified into diploid, polyploid and aneuploid patterns.
All of normal duct epithelia showed diploidy. Polyploid patterns a observed in 3 (10%) lesions of groups I-II,17 (56.7%) of group III 14 (58.4%) of group IV,7 (50%) of group V,and 6 (26.1%) of in vasive carcinomas, and aneuploid patterns were observed in 0%, 10%, 33.3 50% and 73.9%, respectively. This distribution of ploidy patterns re vealed a gradual shift to the main ploidy from diploid to polyploid followed by aneuploid in proportion to the increase of the degree of epithelial atypia.
The frequencies of polyploid cells in each lesiong were determined. Their averages were 0.2% in group I-II,1.9% in group III,3.4% in group IV,4.4% group V,and 6.7% in invasive carcinoma. The S・G_2M phase fractions were Significantly higher in proliferative epithelia than in normal. The results of the study suggest that duct epithelial proliferations of the pancreas have "go instability" leading to a serial clonel evolution and play a significant role in the progressions of pancreatic duct cell carcinoma.
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