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1995 Fiscal Year Final Research Report Summary

Analysis of metastasis or morphology related genes in gastrolintestinal carcinoma

Research Project

Project/Area Number 05671069
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

MORI Masaki  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY ASSOCIATE PROFESSOR, 生体防御医学研究所, 助教授 (70190999)

Co-Investigator(Kenkyū-buntansha) MIMORI Koshi  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,PRESIDENT, 生体防御医学研究所, 医員
Project Period (FY) 1993 – 1995
KeywordsGastrointestinal carcinoma / cDNA subtractioin / Malignant potential / Metastasis / Histology / Genes / Polymerase chain reaction(PCR) / messenger RNA
Research Abstract

We have used the technique of subtractive complementary DNA(cDNA)cloning to dtermine potential new markers for human gastrointestinal carcinomas. By generating subtraction cDNA libraries from human colon cancer tissue minus that from normal colonic mucosa or hepatocellular carcinoma minus norma liver, we demonstrated that the messenger RNA(mRNA)of ribosomal phosphoprotein and that ofprothymosin-alpha were overexpressed in human colorecatal carcinomatous tissue compared to that from adjacent normal colon mucosa. Conversely, we also demostrated that the mRNA of carbonic anhydrase I was overexpressed in tissue from normal colon mucosa compared to that from colorectal carcinomas.
We have recently generated a subtracted cDNA libray from human gastric cancer tissue minus that from normal gastric mucosa from the same patient. One of the overexpressed clones showed 96% identity with matrix metalloproteinase-7(MMP-7).MMP-7 is one of the family members of the matrix-degarding metalloproteinases(MMPs)that may play an important role in invasion and metastasis of cancer cells.
We examined the expression of MMP-7 mRNA in different stages of human colorectal carcinomas.The mRNA signal was greater in the colorectal carcinoma that in paired adjacent normal colonic or rectal mucosa in 39 of 47 cases. The expression of MMP-7 mRNA in tumor tissues increased with increasing Dukes'stage(p<0,05).The MMP-7 mRNA expression was greatest in the matastatic liver lesions. The findings imply that 1)MMP-7 mRNA is overexpressed in human colorectal carcinomas, and2)MMP-7 may prove useful as a marker of biological aggressiveness.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Mori M. et al.: "Significance of carbonic anhydrase expression in human colorectal cancer." Gastroenterol. 105. 820-826 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori M. et al.: "Prothymosin-α mRNA expression correlates with that of c-myc in human colon cancer." Oncogene. 8. 2821-2826 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori M. et al.: "Overexpression of matrix metalloproteinase 7 mRNA in human colon carcinomas." Cancer. 75. 1516-1519 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inoue H, Mori M. et al.: "Expression of tumor rejection antigen "MAGE" gene in human gastric carcinoma." Gastroenterol. 109. 1522-1525 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori M. et al.: "Detection of cancer micrometastases in lymph nodes by the reverse transcriptase-polymerase chain reaction." Cancer Res. 55. 3417-3420 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Begum NA, Mori M. et al.: "Differential display and integrin alpha 6 messenger RNA overexpression in hepathocellular carcinoma." Hapatology. 22. 1447-1455 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori M.et al.: "Significance of carbonic anhydrase expression in human colorectal cancer." Gastroenterol. 105. 820-826 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori M.et al.: "Prothymosin-alpha mRNA expression correlates with that of c-myc in human colon cancer." Oncogene. 8. 2821-2826 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori M.et al.: "Overexpression of matrix metalloproteinase 7 mRNA in human colon carcinomas." Cancer. 75. 1516-1519 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue H,Mori M.et al.: " Expression of tumor rejection antigen"MAGE" gene in human gastric carcinoma." Gastroenteol. 104. 1522-1525 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori M,Mimori K.et al.: "Detection of cancer micrometastases in lymph nodes by the reverse transcriptase-polymerase chain reaction." Cancer Res.55. 3417-3420 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Begum NA,Mori M.et al.: "Differential display and integrin alpha 6 messenger RNA overexpression in hepathocellular carcinoma." Hepatology.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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