• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1994 Fiscal Year Final Research Report Summary

The stimulative effect of macrophages and the influence of cytokines on hepatocyte

Research Project

Project/Area Number 05671077
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionSapporo Medical College School of Medicine

Principal Investigator

HIRATA Koichi  Sapporo Medical University School of Medicine, First Department of Surgery, Professor, 医学部, 教授 (50136959)

Co-Investigator(Kenkyū-buntansha) KATSURAMAKI Tadashi  Sapporo Medical University School of Medicine, First Department of Surgery, Inst, 医学部, 助手 (50253993)
MUKAIYA Mitsuhiro  Sapporo Medical University School of Medicine, First Department of Surgery, Inst, 医学部, 助手 (00253998)
KIMURA Hiromichi  Sapporo Medical University School of Medicine, First Department of Surgery, Inst, 医学部, 助手 (20186331)
DENNO Ryuichi  Sapporo Medical University School of Medicine, First Department of Surgery, Assi, 医学部, 講師 (40163943)
KOBAYASHI Kenji  Sapporo Medical University School of Medicine, First Department of Surgery, Assi, 医学部, 講師 (30153604)
Project Period (FY) 1993 – 1994
KeywordsCholestasis / Endotoxin / Macrophage / Cytokine / Kupffer cell / Hepatocyte
Research Abstract

Reactive products between lipopolysaccharide and Kupffer cells or polymorphonuclear cells were proven to have a characteristic effect on secretion of bile and on function of bile canaliculus. Experimentally, decrease of bile flow and bile acids secretion, lysosomal activations, mitochondrial disturbances and the morphological changes of bile canalidulus are confirmed. In order to clarify above phenomena, monoclonal antibodies for one fraction of culture media between lipopolysaccharide and macrophages were studied. None among several monoclonal a ntibodies gained had significant anti-effects for the functions of that fraction. The relationships between those antibodies and the well-known cytokines, i.e.IL-1, IL-6, IL-8, IL-10, TNF and PAF,were made sure and no-specific correlation was observed. Namely, this monoclonal antibody is not specific for those cytokines. Howerver, one of them has an anti-effect of hepatocyte cytotoxicity by reactive product from cultured media. In details abou … More t this antibody and antigen are in study. The administration experiment via the portal vein was observed in vivo. And also in vitro, the effect of this antibody had been compared to that of the commercial antibodies for well-known cytokines about cytotoxicity and modulationin rat hepatocytes. The highly specific phenomena could not be found in a monoclonal antibody produced in this series, therefore, the commercial antibodies were utilized in most of in vivo experiment. The pretreatments by those antibodies had significant effects of total bile flow, the changes of bile acid concentrations and the morphological changes of bile canaliculus but no effect is obseved in the posttreatment following the perfusion of reactive products.
The significant phenomena using by antibodies were observed in "in vitro" experiment than in "in vivo", on the point of views, i.e.the activation of hepatocyte lysosomal enzymes and the decrease of hepatocyte mitochondrial ATPase by the reactive products. The characteristics of an unknown monoclonal antibody and its antigan is recognized to be important and will be continued to study in this research. Less

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Hirata et al: "The roles of macrophage and Sinusoidal endothelial cells on hepatic failure." Therapeutic Research. 15. 1866-1875 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桂巻正: "術後胆汁うっ滞,肝機能に及ぼすPGE,持続的静脈内投与の効果" 現代医療. 24. 3481-3488 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平田公一: "敗血症と肝障害-sinusoidal cellsの関与を中心に-" 集中治療. 6. 235-246 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平田公一: "間質構成の制御機序-サイトカイン-" Biomedical Penspectives. 3. 19-24 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平田公一: "侵襲と肝内免疫担当細胞類洞構成細胞反応" Surgical Trauma and Immunological Response. 3. 44-48 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山口浩史: "敗血症による肝障害の成因、特に類洞構成細胞変化と類洞循環障害について" 日本腹部救急医学会雑誌. 14. 1017-1026 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平田公一: "外科医臨床ハンドブック(1)周術期感染の対策" 中山書店, 6 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirata, K., Ohmura, T., Yamaguchi, H., Furuhata, T., Yamashiro, K., Katsuramaki, T., Mukaiya, M., Denno, R.: "The roles of macrophage and sinusoidal endothelial cells on hepatic failure." Therapeutic Research. 15. 1866-1875 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuramaki, T., Furuhata, T., Mukaiya, M., Oikawa, I., Kimura, H., Denno, R., Hirata, K.: "The effects of continuous intravenous administration of PGE,on postoperative cholestasis and liver function." Modern Medicine. 24. 3481-3488 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirata, K., Yamashiro, K., Yamaguchi, K., Denno, R., Xion, M., Matsuno, T.: "Sepsis and hepatic dysfunction. -the relationship of the sinusoidal cells-" Intensive and critical care medicine.6. 235-246 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirata, K., Yagihashi, A., Yamaguchi, K.: "The control mechanism of interstitial constitution-Cytokines-." Biomedical Perspectives. 3. 19-24 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirata, K., Yamaguchi, K., Ohmura, T., Ezoe, E., Yamashiro, K., Katsuramaki, T., Mukaiya, M., Denno, R., Xion, M., Matsuno, T., Kimura, H.: "Surgical trauma and the reactions of intrahepatic immune cells and of sinusoidal lining cells." Surgical trauma and immunological response. 3. 44-48 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi, K., Hirata, K., Yamashiro, K., Mukaiya, M., Katsuramaki, T., Ohmura, T., Xion, M: "Cause of hepatic disturbance by sepsis. Especially about the changes of sinusoidal lining cells and the insufficiency of sinusoidal circulation." Journal of Abdominal Emergency Medicine. 14. 1017-1026 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirata, K., Kimura, H., Takahashi, K.: The control of severe infection. 1.the control of sepsis. In "The Clinical Handbook for Surgeons (1) The control of perioperative infection. "edited by Idezuki, Y.Nakayama Lib. Corp., Tokyo, 244-249 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi