1994 Fiscal Year Final Research Report Summary
BASIC STUDY FOR SELECTIVE GENE TARGETING THERAPY OF COLON CANCER USING MONOCLONAL ANTIBODY
| Project/Area Number |
05671080
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | KYOTO PREFECTURAL UNIVERSITY OF MEDICINE |
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Principal Investigator |
YAMAGUCHI Toshiharu KYOTO PREFECTURAL UNIVERSITY OF MEDICINE,MEDICAL SCHOOL,INSTRUCTOR, 医学部, 講師 (90111327)
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| Project Period (FY) |
1993 – 1994
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| Keywords | ANITSENSE DNA / TARGETING THERAPY / GENE THERAPY / ANTIBODY |
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| Research Abstract |
IN 1993, THE TRIAL TO APPLY MONOCLONAL ANTIBODY BOUND ANTISENSE DNA FOR SELECTIVE IMMUNOTARGETING GENE THERAPY OF COLON CANCER HAD BEEN CONDUCTED, THOUGH THE RESULTS WERE NOT SATISFACTORY.IN 1994, ANTISENSE DNA INTORDUCED INTO RETRO-VIRUS THOSE WERE COATED WITH MONOCLONAL ANITOBODY AGAINST COLON CANCER HAD BEEN DEVELOPED.THE HUMAN COLON CANCER CELL LINES WERE INFECTED AND THE EXPRESSION OF c-myc GENE WAS EXAMINED.THE EXPRESSION OF c-myc GENE WAS INHIBITED IN ANTIGEN POSITIVE CANCER CELL LINES BUT NOT IN CELL LINES WITHOUT ANTIGEN.THE SIZE OF MONOCLONAL ANTIBODY CHANGED THE AFFINITY OF ANTIBODEY RESULTED IN DECREASED EXPRESSION OF ANTISENSE DNA IN TARGET CELLS.TEH MOST IMPORTANT PROBRLEM TO OVERCOME SEEMED POOR EFFEICENCY OF VIRUS INFECTION.
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