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1994 Fiscal Year Final Research Report Summary

Experimental Study of a Hybrid Artificial Esophagus Using Cultured Human Esophageal Epithelial Cells.

Research Project

Project/Area Number 05671085
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionKeio University

Principal Investigator

ANDO Nobutoshi  Keio Univ.Sch.Med.Assistant Professor, 医学部, 講師 (90101972)

Co-Investigator(Kenkyū-buntansha) TAMURA Akihiko  Keio Univ.Sch.Med.Assistant, 医学部, 助手 (00227274)
KAZUYUKI Tujituka  Keio Univ.Sch.Med.Assistant, 医学部, 助手 (70227403)
SHINOZAKI Hiroharu  Keio Univ.Sch.Med.Assistant, 医学部, 助手 (40235544)
Project Period (FY) 1993 – 1994
KeywordsArtificial Esophagus / Esophageal Epithelial cell / Fibroblast / Cell Culture / Collagen Gel / Transplantation / Muscle Flap / Hybrid Organ
Research Abstract

An artificial esophagus has been reconstructed by cell culture method. It is important to line inner side of the artificial esophagus with epithelium to prevent strictures and reinforce the lumen. Normal human epithelial cells were cultured on the collagen in which polyglycolic acid (PGA) mesh was embedded. 7 days later, atube was made by suturing the edge of PGA mesh in vitro. The tube consisting of human epithelium and collagen-PGA mesh was transplanted on the surface of alattisimus dorsi muscle flap of an athymic rat. On the 20th day after transplantation, the luminal structure was maintained without strictures.
To accelerate stratification of the esophageal epithelial cells, they were cultured on a collagen sheet in which 0,4 and 8*10^5 cells/ml of human fibroblasts were already embedded respectively. They were examined histologically 3 weeks later. Though human epithelial cells on a collagen sheet without fibroblasts stratified 2-3 fold, on collagen in which fibroblasts 4*10^5 cells/ml were embedded, they stratified 4-5 fold. Further fibroblasts 8*10^5 cells/ml were embedded in, they stratified 17-18 fold and differentiated much more.
The sheet consisting of human epithelium, fibroblasts 4*10^5 cells/ml and collagen was transplanted on a surface of the abdominal rectus muscle of an athymic rat. It was examined histologically 2 weeks later. Angiogenesis from the rectus muscle into the collagen sheet and more than 10 fold stratification of the epithelium were observed.
Stratification of the human epithelial cells were accelerated by the fibroblasts. The fibroblasts are very useful for stratification of inner side of the artificial esophagus eventually.

  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Michio Sato: "Experimental Study of a Hybrid Artificial Esophagus Using Cultured Human Esophageal Epithelial Cells" ASAIO Journal. 39. 554-557 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤 道夫: "ハイブリッド型人工食道内腔上皮化のための実験的研究" Progress in Medicine. 13. 212-213 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Michio Sato: "An Artificial Esophagus Consisting of Cultured Human Esoghageal Epithelial Cells,Polyglycelic Acid Mesh,and Collagen" ASAIO Journal. 40. 389-392 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤 道夫: "培養ヒト食道上皮細胞を用いた組織再構築型人工食道の実験的研究" Progress in Medicine. 14. 260-261 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三木 浩栄: "細胞培養法を応用した組織再構築型人工食道-線維芽細胞による食道上皮細胞重層化の促進作用-" Progress in Medicine. 15. (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Michio, Sato: "Experimental Study of a Hybrid Artificial Esophagus Using Cultured Human Esophageal Epithelial Cells." ASAIO Journal. 39. 554-557 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Michio Sato: "AN Artificial Esophagus Consisting of Cultured Human Esophageal Epithelial Cells, Polyglycolic Acid Mesh, and Collagen." ASAIO Journal. 40. 389-392 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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