1994 Fiscal Year Final Research Report Summary
Radioimmunodetection of recurrence of colorectal cancer using monoclonal antibody
| Project/Area Number |
05671097
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Fujita Health University |
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Principal Investigator |
WATANABE Tadashi Fujita Health Univ.Professor, 医学部, 教授 (20144157)
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| Project Period (FY) |
1993 – 1994
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| Keywords | radioimmunodetection / colorectal cancer / monoclonal antibody / carcinoembryonic antigen |
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| Research Abstract |
Imaging using radiolabeled monoclonal antibody (MAb) to tumor-associated antigen is potentially specific approach to qualitative diagnosis. The MAbCEA102 against CEA was produced by immunization with purified CEA,and used as a radiotracer for the imaging of colorectal cancer. To investgate the specific accumulation of radiolabeled CEA102, autoradiographic study using Fuji Computed Radiography (FCR) was performed, and predominant localizations of 131-I-labeled-CEA102 were observed in primary tumors and even small liver metastases. We then applied 131-I-labeled-CEA102 to 20 cases with liver metastases, local recurrences, and/or primary tumors. Overall sensitivity was 71.4%, and no false positive was observed. Since 131-I has energic gamma-ray emission, and high accumulation of free 131-I in bladder, we attempted using 111-In as a radiotracer which has a short half life and provides to use Single Photon Emission CT (SPECT) . We applied to 10 cases (primary tumor 1, CEA producing tumor of ileum 1, and local recurrence of rectal cancer 8) using 111-In-labeled CEA102. Overall sensitivity was 70%. Although this radiotracer was not suitable to detect liver metastasis due to high accumulation of 111-In in liver, it was especially useful to diagnose local recurrence of rectal cancer using Emission CT.The above results demonstrated that radioimaging has a great potential in qualitative diagnosis.
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