1994 Fiscal Year Final Research Report Summary
Significance of levels of serum keratan sulfate
| Project/Area Number |
05671240
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kinki University |
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Principal Investigator |
TANAKA S Dept.Orthopaedic Surgery, Kinki University, Professor, 医学部, 教授 (00026840)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA F Dept.Orthopaedic Surgery, Kinki University, assistant, 医学部, 助手 (00248014)
FUKUDA K Dept.Orthopaedic Surgery, Kinki University, lecturer, 医学部, 講師 (50201744)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Keratan sulfate / Articular cartilage / Osteoarthritis |
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| Research Abstract |
Keratan sulfate (KS) is glycosaminoglycan and specifically distributed in the articular cartilage.We established the assay system and reported the alterations of serum keratan sulfate in patients of arthrosis.Our goal in this project is define the significance of serum KS levels.
To solve this problem, we first established the assay system to evaluate the cartilage degradation.Bovine articular cartilage was cultured in the presence of interleukin-1 (IL-1) and levels of glycosaminoglycans released into the media were measured.KS,chondroitin sulfate and hyaluronic acid were released with IL-1, time-and dose-dependent manner (Jpn.J.Rheumatology, in press).
We also found that superoxide anion plays an important role of maytix degration (Agents Actions, 1995).Inhibition of matrix synthesis is also important factor of matrix degradation of articular cartilage.Our KS assay system revealed the role of prostaglandin E2 (Inflammation Res.in press).
Our data obtained in this year clearly indicates that the levels of KS reflect both destruction and inhibition of cartilage matrix.
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