1995 Fiscal Year Final Research Report Summary
Assessment of sperm function by using confocal laser microscopy
| Project/Area Number |
05671316
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Tottori University |
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Principal Investigator |
MIYAGAWA Ikuo INSTITUTION,DEPARTMENT,TITLE OF POSITION TOTTORI UNIVERSITY FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (60006781)
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| Co-Investigator(Kenkyū-buntansha) |
SOFIKITIS Nikolaos TOTTORI UNIVERSITY FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 助手 (60252863)
HIRAKAWA Shinji INSTITUTION,DEPARTMENT,TITLE OF POSITION TOTTORI UNIVERSITY FACULTY OF MEDICINE,, 医学部, 助教授 (00181149)
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| Project Period (FY) |
1993 – 1995
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| Keywords | spermatozoom / laser microscopy / fartile potential / acrosin activity / HOST / male infertility / mid-piece |
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| Research Abstract |
We have used semen analysis to evaluate sperm function. However, this parameter is not much correlated with fertile potential.
we evaluated sperm morphometric parameters of idopathic infertile men and infertile men with valicocele by using confocal laser scanning microscopy and assested whether these parameters measured by this method were correlated with acrosin activity, HOST (hypoosmotic swelling test) , and fertile potential.
We concluded as follows.
1) The length of sperm mid-piece, ratio of acrosomal region of sperm head, acrosin activity, and HOST is significantly different between fertile men and infertile men.
2) The length of sperm mid-piece and the ratio of acrosomal region of sperm head are much correlated with the percentage of sperm motility and acrosin activity, respectively. Inaddition, the percentage of normal morphometric spermatozoa is much correlated with the fertile potential.
3) Values of sperm morphometric parameters after varicocelectomy can reliably predict sperm fertilizing potential.
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[Publications] Nikolaos V. Sofikitis, M. D., Ph. D. ‡§ Toshiko Toda. M. D., Ph. D. ¶ Ikuo Miyagawa, M. D., Ph.D. ‡ Akihiro Iino, M. D., Ph.D. ^<**> Panaviotis M. Zavos, Ed. S., Ph. D. || Naoki Terakawa, M. D., Ph. D. ¶: "Confocal scanning laser microscopy of morphometric human sperm parameters: correlation with acrosin profiles and fertilizing capacity^*+" FERTILITY AND STERILITY. 62. 376-386 (1994)
Description
「研究成果報告書概要(和文)」より
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