• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1994 Fiscal Year Final Research Report Summary

Analysis of Biofilm Disease as Cryptic Bacterial Infection and Study for Medical Treatment

Research Project

Project/Area Number 05671318
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionOkayama University

Principal Investigator

FUJINAGA Yukako  Okayama Univ.Med.School Research Associate, 医学部, 助手 (60252954)

Co-Investigator(Kenkyū-buntansha) TOMOCHIKA Kenichi  Okayama Univ.Pharm.Sciences Assistant Prof., 薬学部, 助教授 (00093691)
Project Period (FY) 1993 – 1994
KeywordsBiofilm / Urinary truct infection / Confocal laser scanning microscope / P.aeruginosa / Exopolysaccharide / Antimicrobial agent
Research Abstract

Pseudomonas aeruginosa is commonly found in aquatic environments, including rivers, sewerage, and the sinks of hospital wards. To counteract adverse environmental conditions, it is known that P.aeruginosa survives by producing a exopolysaccharide that forms a biofilm. Recently, some bacterial cells growing as a biofilm have been detected in many types of chronic persistent infection.
During this project, we examined the condition of biofilm formation onto catheter materials using artificial bladder connected with modified Robins device and observed the formation of biofilm with scanning electronmicroscope and confocal laser scanning microscope. The biofilm formation in artificial bladder system depended on the flow rate of urine at the initial step of bacterial adherence. On the observation of biofilm, many researchers used the scanning electronmicroscope, but this method required the fixation and dehydoration of biofilm. To observe the structure of native biofilm, confocal laser scanning microscope (CLSM) was used. After the examination of staining method, acridine orange and safranine were used for the bacterial staining and FITC conjyugated ConA was used for the staining of exopolysaccharide. Effects of antimicrobial agent on formation and degradation fo biofilm were examined by CLSM.By these observations, conbination of antimicrobial agents was effective to the destruction of biofilm.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Kumon,H,et al: "A Sandwich Cup Method for the Penetration Assay of Antimicrobial Agent through Pseudomonas Exopolysaccharido" Microbiol.Immunol.38. 615-619 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡辺豊彦他: "共焦点レーザー走査顕微鏡による細菌バイオフィルムの観察-P.aeruginosa biofilmとS.epidermidis biofilmの比較-" Bacterial Adherence研究会. 8(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡辺豊彦他: "共焦点レーザー走査顕微鏡による細菌バイオフィルムの観察" Bacterial Adherence研究会. 7. 111-115 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kumon, H., Tomochika, K., Matunaga, T., Ogawa, M., and Ohmori, H.: "A Sandwich Cup Method for the Penetration Assay of Antimicrobial Agents through Pseudomonas Exopolysaccharides." Microbiol.Immunol.38 (8). 615-619 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1996-04-15  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi