1994 Fiscal Year Final Research Report Summary
cryopreservation of human semen with male infertility
| Project/Area Number |
05671338
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | TOKYO DENTAL COLLEGE |
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Principal Investigator |
ISHIKAWA Hiromichi Tokyo Dental College, Associate Prof., 歯学部, 助教授 (60112679)
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| Project Period (FY) |
1993 – 1994
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| Keywords | cryopreservation / retrograde ejaculation / osmotic pressure / cryo-accumulation / liquid nitrogen vapor |
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| Research Abstract |
1 To simplifies freezing procedure of human sperm, the floating platform was developed. Sperm-KS-II cryomedium mixture was frozen at-30ﾟC/min, and stored in liquid nitrogen container.
2 Hipper osmotic pressure by glycerin, which involved in KS-II cryomedium, decreased sperm motility, pentoxifilline, a phosphodiesterase inhibitor, protected sperm viability.
3 To recover motile sperm, the contents in bladder was replaced with culture medium prior to retrograde ejaculation. Contamination of urine in the culture medium denatured sperm. Whereas addition of pentoxifilline in the culture medium suppressed the toxicity of urine, improving sperm motility and velocity.
4 Cryo-accumulation of human sperm provide sufficient number of motile sperm for artificial insemination. Its clinical application to infertile couples who suffered from ejaculatory disturbance resulted two successful prehnancies following artificial insemination.
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