1995 Fiscal Year Final Research Report Summary
Effect of testosterone on alpha_1-adrenoceptors in rabbit corpus cavernosum penis
| Project/Area Number |
05671343
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
YAJIMA Michitaka St.Marianna University, Urology Assistant professor, 医学部, 講師 (10230202)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Katsuyuki St.Marianna University, Urology, Instructor, 医学部, 助手 (90267609)
IWAMOTO Teruaki St.Marianna University, Urology Associate professor, 医学部, 助教授 (60046117)
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| Project Period (FY) |
1993 – 1995
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| Keywords | Corpus cavernosum penis / Adrenoceptor / Testosterone / Erectile dysfunction / Rabbit / Erection |
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| Research Abstract |
We have thought that androgen may act on not only central nervous system but also corpus cavernosum penis. Therefore, we have planned this study.
We performed autoradiography using [^3H] prazosin and [^3H] YM 617 to detect and determine the localizatio of alpha_1-adrenoceptors in rabbit penis. The autoradiography demonstrated the presence of alpha_1-adrenoceptor in corpus cavernosum and spongiosum.
We studied effect of androgen on alpha_1-adrenoceptors in corpus cavernosum penis using organ bath method and radiobinding assay. Three groups of mature male rabbits were studied over a similar time span ; control, castration and castration+testosterone replacement groups. The contractility of the corpus cavernosum strips increased significantly in the castration+testosterone replacement group, but decreased significantly in the castration group as compared with the control group after adding norepinephrine, phenylephrine and clonidine. The results of the radiobinding assay demonstrated that the density of alpha_1-adrenoceptors of corpus cavernosum decreased by castraiton and increased by testosterone replacement.
The results of this study suggest that androgen may act on not only central nervous system but also corpus cavernosum penis, and be involved in sexual function especially penile erectin.
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