1995 Fiscal Year Final Research Report Summary
Comparative study of erdometrial carciroma between Japanese women and American women
| Project/Area Number |
05671347
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
SATO Shinji Tohoku University Dept. of OB/GYN,Associate Prof., 医学部, 講師 (10142960)
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| Co-Investigator(Kenkyū-buntansha) |
SASANO Hironobu Tohoku University Dept. of Pathology, Instructor, 医学部, 助手 (50187142)
FUJITA Nobuhiro Tohoku University Dept. of OB/GYN,Instructor, 医学部附属病院, 助手 (20250748)
ITO Kiyoshi Tohoku University Dept. of OB/GYN,Instructor, 医学部附属病院, 助手 (70241594)
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| Project Period (FY) |
1993 – 1995
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| Keywords | P450 aromatase / Endometrial carcinoma / lmmunohistochemistry |
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| Research Abstract |
The expression of P450 aromatase and other steroidogenic enzymes were evaluated in 42 endometrioid endometrial carcinomas, 23 endometrial hyperplasias, and 7 normal endometrial specimens. These findings were correlated with clinicopathological findings to elucidate the possible biological significance of in situ estrogen production in the development of human endometrial carcinoma. Only weak aromatase immunoreactivity was observed in vascular walls and myometrial cells. In contrast, strong aromatase stromal immunoreactivity was observed in 28 of 42(66.7%)endometrial carcinomas. However, no stromal immunoreactivity was seen in normal or hyperplastic endometrial specimens. Immunoreactivity in the carcinoma stromal cells was significantly increased at sites of invasion. These aromatasepositive cells were immunohistochemically negative for other steroidogenic enzymes involved in estrogen biosynthesis. In situ hybridization studies revealed aromatase mRNA hybridization signals in stromal ce
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lls but not in carcinoma cells. The distribution of aromatase mRNA correlated well with the immunohistochemical localization of aromatase anzyme. Quantitation of aromatase activity demonstrated 8.75 <minus-plus> 2.75 pmol/hour/mg of protein for endo-metrial carcinomas (22 specimens) and 0.98 <minus-plus> 1.95 pmol/hour/mg of protein for normal endometrial specimens(4 specimens). Aromatase activity was found in both estrogen receptor-positive and -negative endometrial carcinoma. Aromatase did not vary with respect to the menopausal status of patients with endometrial carcinoma. These results suggest that estrogen is produced in situ in endometrial carcinoma but not in benign endometrial lesions. Such locally synthesized estrogen may act on carcinoma cells in a paracrine fashion to promote tumor growth. Additional inverstigations are necessary, but increased aromatase expression in the stromal cells of endometrial carcinoma may therefore play an important role in the development of human endometrioid endometrial carcin Less
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