1994 Fiscal Year Annual Research Report

卵巣におけるPACAP作用機構の機能的組織学的検討

Research Project

Project/Area Number	05671351
Research Institution	University of Tsukuba
Principal Investigator	臼杵さとし筑波大学, 臨床医学系, 助教授 (50092151)
Co-Investigator(Kenkyū-buntansha)	塩田正樹東京大学, 医学部, 助手 (80134526) 臼杵祥江筑波大学, 臨床医学系, 講師 (80134229)
Keywords	PACAP-27 38 / PACAP Receptor / ERAANPS / Endothelin Receptor / Angiotensin Receptor / DNA Polymerase α / FSH / Growth Hormone
Research Abstract	Secretin/glucagon/vasoactive polypetide family petidesのMemberであるpituitary adenylate cyclase-activating polypetide(PACAP)のPACAP-38およびPACAP-27が卵巣に存在し、主にTypeII PACAP receptorを介して卵巣の機能を種々に調節することが明らかとなった(Type I PACAP receptorの存在も否定できない)。PACAP-38はCyclic adenosine 3',5'-monophosphateやCytosolic Ca^<2+>の増加にも関与し、Follicle-stimulating hormoneとDependentないしIndependentに卵巣のプロゲステロン,エストロゲン,特にプロゲステロンを増加させる。Deoxyribonucleic acid(DNA)複製酵素であるDNA Polymerase α活性も調節しているようである。さらに、エンドセリンレセプターないしアンジオテンシンレセプターを調節している可能性も示唆され、卵胞の閉鎖にも深い関与するとともに、卵の機能を抑制することが示唆される。Endothelin-renin-angiotensin-atrial natriuretic peptide system(ERAANPS)系やGrowth hormoneの作用にも関与しているらしい。このように、PACAPはAutocrineないしParacrine的に種々のホルモン系と深く関わり、卵巣の機能を調節することが明らかとなった。研究は概略的には計画的に進んでいる。

Research Products

(8 results)

All Other

All Publications (8 results)

[Publications] Satoshi Usuki,et al.: "Angiotensin 2 Receptor Type 2 and ETB-R in Rat Ovarian Granulosa Cells" New Achievements in Research of Ovarian Function,Ares Serono Symposia Publications,Rome. (in press).
[Publications] Masami Tanaka,et al.: "Characterization of Angiotensin II Receptor Type 2 during Differentiation and Apoptosis of Rat Ovarian Cultured Granulosa Cells" Biochem.Biophys.Res.Commun.(in press).
[Publications] Junji Ohnishi,et al.: "Effect of Dithiothreitol on Angiotensin II Receptor Type II in Rat Ovarian Cultured Granulosa Cells" Biochimica et Biophysica Acta. 1192. 286-288 (1994)
[Publications] Satoshi Usuki,et al.: "Intraovarian Endothelin-renin-angiotensin-atrial Natriuretic Peptide System" Studies in Profertility Series Ovarian Endocrinopathies. 2. 77-84 (1994)
[Publications] Satoshi Usuki,et al.: "Tokishakuyakusan Enhances DNA Polymerase α Activity in Relationship to the LH/FSH Surge in Rats" Am.J.Chin.Med.(in press).
[Publications] Satoshi Usuki,et al.: "There Exist Two Types of Endothelin Receptors,ETA and ETB,and ETB Exceeds ETA in Cultured Rat Ovarian Grabilosa Cells" J.Jpn.Comp.Endocrinol.9. 63 (1994)
[Publications] 臼杵,ほか: "エンドセリンと性周期および妊娠分娩" 最新医学. 49. 46-59 (1994)
[Publications] Satoshi Usuki,et al.: "Endothelin Vasoactive Peptide,Levels and Its Relationship to Various Hormones in Blood or Urine of The Normal Women" Hypertension. (in press).

1994 Fiscal Year Annual Research Report

卵巣におけるPACAP作用機構の機能的組織学的検討

Principal Investigator

臼杵 さとし 筑波大学, 臨床医学系, 助教授 (50092151)

Research Products

[Publications] Satoshi Usuki,et al.: "Angiotensin 2 Receptor Type 2 and ETB-R in Rat Ovarian Granulosa Cells" New Achievements in Research of Ovarian Function,Ares Serono Symposia Publications,Rome. (in press).

[Publications] Masami Tanaka,et al.: "Characterization of Angiotensin II Receptor Type 2 during Differentiation and Apoptosis of Rat Ovarian Cultured Granulosa Cells" Biochem.Biophys.Res.Commun.(in press).

[Publications] Junji Ohnishi,et al.: "Effect of Dithiothreitol on Angiotensin II Receptor Type II in Rat Ovarian Cultured Granulosa Cells" Biochimica et Biophysica Acta. 1192. 286-288 (1994)

[Publications] Satoshi Usuki,et al.: "Intraovarian Endothelin-renin-angiotensin-atrial Natriuretic Peptide System" Studies in Profertility Series Ovarian Endocrinopathies. 2. 77-84 (1994)

[Publications] Satoshi Usuki,et al.: "Tokishakuyakusan Enhances DNA Polymerase α Activity in Relationship to the LH/FSH Surge in Rats" Am.J.Chin.Med.(in press).

[Publications] Satoshi Usuki,et al.: "There Exist Two Types of Endothelin Receptors,ETA and ETB,and ETB Exceeds ETA in Cultured Rat Ovarian Grabilosa Cells" J.Jpn.Comp.Endocrinol.9. 63 (1994)

[Publications] 臼杵,ほか: "エンドセリンと性周期および妊娠分娩" 最新医学. 49. 46-59 (1994)

[Publications] Satoshi Usuki,et al.: "Endothelin Vasoactive Peptide,Levels and Its Relationship to Various Hormones in Blood or Urine of The Normal Women" Hypertension. (in press).

臼杵さとし筑波大学, 臨床医学系, 助教授 (50092151)