1994 Fiscal Year Final Research Report Summary
Development of a novel assay system of transplacental transport and study of fetal growth mechanism
| Project/Area Number |
05671361
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagoya University |
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Principal Investigator |
KURAUCHI Osamu School of Medicine, Nagoya University, 医学部, 講師 (80195528)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUTANI Shigehiko Associate Professor, School of Medicine, Nagoya University, 医学部, 助教授 (00159162)
MURATA Yoshiharu Associate Professor, Research Institute of Environmental Medicine, Nagoya Univer, 環境医学研究所, 助教授 (80174308)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Transwell / Transport / Fetal liver / HGF / Amniotic fluid / Amnion / Cell proliferation / fetal lung |
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| Research Abstract |
The trophoblast derived cells were cultured on a polycarbonate membrane of 0.4mum pores with medium on each side. The cells could electroscopically form a continuous cellular sheet over the polycarbonate membrane of Transwell. However, the permeability study suggested that this cell layr possesses a significant leak that must be controlled for. The results suggest that the transfer of material through the intercellular lateral space must be considered when the study of transtrophoblast transfer is performed using cell monolayr.
Hepatocyte growth factor (HGF) was measured in 28 samples of amniotic fluid, 1 of fetal urine and 5 of first neonatal urine. The mean level of HGF was 12.4<plus-minus>4.5 ng/ml (second trimester) and 10.5<plus-minus>6.6 ng/ml (third timester). These values were extremely high compared to that in plasma from normal subjects and greater than the plasma levels from patients with acute hepatitis. The concentration of amniotic HGF at second trimester showed a significant inverse correlation both with birth weight (r=0.47 ; p<0.05) and birth weight deviation(r=0.54 ; p<0.02). HGF stimulated DNA synthesis of human fetal liver cells in vitro. While the effect was dose dependent, a maximal response was reached with about 0.2ng/ml, attaining a 1.3-fold stimulation. Immunohistochemical study showed positive cytoplasmic staining for HGF in the cell of amnion, whereas no staining was observed in the cells of chorion and decidua. Intense immunostaining for HGF receptor in the fetal lung was observed specifically in bronchial epithelial cells. The present results suggested that the ingestion of amniotic fluid into the lung of fetus might promote growth and differentiation by way of HGF in amniotic fl
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