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1995 Fiscal Year Final Research Report Summary

Research for regulation of expression of specific proteins in human granulosa cells.

Research Project

Project/Area Number 05671362
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionNagoya University

Principal Investigator

ASADA Yoshimasa  Nagoya Univ.School of Medicine, Associate Prof., 医学部, 講師 (70231884)

Co-Investigator(Kenkyū-buntansha) SUGANUMA Nobuhiko  Nagoya Univ.School of Medicine, Associate Prof., 医学部, 助教授 (30179113)
KONDO Ikuyo  Nagoya Univ.School of Medicine, Assistant Prof., 医学部, 助手 (40215447)
Project Period (FY) 1993 – 1995
KeywordsInhibin / Activin / Follistatin / Gene expression / Granulosa cell / Northern blotting
Research Abstract

To analyze direct effects of estrogen on gene expressions of inhibin subunits in vitro, the mRNA levels of inhibin alpha- and betaA-subunits were measured in rat granulosa cells cultured with FSH or estradiol. Through the culture process of the granulosa cells with serum-free medium, both the alpha- and betaA subunit mRNAs decreased, which were partially rescued by addition of FSH to the culture medium. To examine whether these FSH effects is mediated via estrogen production, estradiol and/or tamoxifen, one of anti-estrogenic drugs, were added to the cultured granulosa cells. After 36-hour culture with estradiol, the inhibin alpha-subunit decreased whereas the inhibin betaA-subunit increased, with dose-responsive manner. Tamoxifen showed a completely reversed effects to estradiol, and combination of estradiol and tamoxifen resulted similar levels of inhibin-alpha and -betaA mRNAs with control. These results indicated that estrogen would have a certain pathway to affect gene expressions of the inhibin subunits in the rat granulosa cells, and may regulate the production of inhibin and activin through paracrine system.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Kenji Furui: "Identification of two point mutations in the gene coding luteinzing hormone(LH) β-subnniz,associated with immunologically anomalous LH variants" J.Clin.Endocrinol.Metab.78. 107-113 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤智子: "着床における自己抗体の意義-体外受精胚移殖周期における検討" 日本受精着床学会詬. 11. 69-72 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuhiko Suganuma: "Screening of the mutations in luteinizing hormone β-subnnit in the patients with menstrual disorlers" Fertil.Steril.63. 989-995 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 菅沼信彦: "遺伝子組み換えの産婦人科領域への応用" 日産婦詬. 47. 1189-1193 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 菅沼信彦: "遺伝子組み換えの産婦人科への応用-その現況と将来-" 産婦人科治療. 71. 553-557 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furui K,Suganuma N,Tsukahara S,Asada Y,Kikkawa F,Tanaka M,Ozawa T,Tomoda Y: "Identification of two point mutations in the gene coding luteinizing hormone (LH) beta-subunit, associated with immunologically anomalous LH variants." J Clin Endocrinol Metab. 78. 107-113 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ando T,Sun JR,Tsukahara S,Kitagawa T,Moriwaki T,Furui K,Kondo I,Suganuma N: "The role of autoantibodies in implantation of embryos. (Japanese)" Journal of Fertilization and Implantation [Tokyo]. 11. 69-72 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suganuma N,Furui K,Furuhashi M,Asada Y,Kikkawa F,Tomoda Y: "Screening of the mutations in luteinizing hormone beta-subunit in the patients with menstrual disorders." Fertil Steril. 63. 989-995 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suganuma N: "Application of molecular biological techniques for obstetric and gynecologic Field. (Japanese)" Acta Obstet Gynaec Jpn. 47. 1189-1193 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suganuma N: "Application of Molecular Biological Techniques for obstetrics and gynecology-present and future. (Japanese)" Obstetrical and Gynecological Therapy. 71. 553-557 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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