1994 Fiscal Year Final Research Report Summary
Research on the clonal analysis of gynecologic tumors
| Project/Area Number |
05671368
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
SAWADA Masumi Staff of Department of Obstetrics and Gynecology, Osaka University Medical School, 医学部, 助手 (60226074)
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| Co-Investigator(Kenkyū-buntansha) |
AZUMA Chihiro Staff of Department of Obstetrics and Gynecology, Osaka University Medical Schoo, 医学部, 助手 (20151061)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Clonality / PGK gene / Gynecologic cancer / Uterine leiomyoma / PCR / RFLP |
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| Research Abstract |
To investigate the clonality of human gynecologic tumors, small DNA samples prepared from cryostat sections were analyzed by means of the polymerase chain reaction (PCR). The method used for clonal analysis was based on restriction fragment length polymorphism of the X-chromosome-linked phosphoglycerokinase (PGK) gene and on the differential methylation of the PGK gene due to random inactivation of 1 of 2 X-chromosomes by methylation in females. Among 76 gynecologic tumors tested, 34 were found to be heterozygous for the BstXI polymorphism of the PGK gene. All the 25 gynecologic cancers (4 cervix, 11 endometrium, 7 ovary and 3 fallopian tube) and 22 uterine leiomyomas analyzed by the PCR-based method were monoclonal in origin while adjacent normal tissues were polyclonal. When DNA samples were prepared from widely separated sites of tumors and/or metastatic lesions, every sample was found to be monoclonal, and the same allele of that PGK gene was inactivated in each case. All leiomyoma samples consisted of single type of inactive allele, even though alleles were detected that were specific to each nodule indicating the leiomyoma nodules were unicellular in origin but generated independently. These results demonstrate that clonal analysis by PCR offers a good method for studying clonality in small DNA samples prepared from cryostat sections of tumors. This method could be applied to distinguish between benign and malignant gynecologic lesions.
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