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1994 Fiscal Year Final Research Report Summary

Research on the clonal analysis of gynecologic tumors

Research Project

Project/Area Number 05671368
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionOsaka University

Principal Investigator

SAWADA Masumi  Staff of Department of Obstetrics and Gynecology, Osaka University Medical School, 医学部, 助手 (60226074)

Co-Investigator(Kenkyū-buntansha) AZUMA Chihiro  Staff of Department of Obstetrics and Gynecology, Osaka University Medical Schoo, 医学部, 助手 (20151061)
Project Period (FY) 1993 – 1994
KeywordsClonality / PGK gene / Gynecologic cancer / Uterine leiomyoma / PCR / RFLP
Research Abstract

To investigate the clonality of human gynecologic tumors, small DNA samples prepared from cryostat sections were analyzed by means of the polymerase chain reaction (PCR). The method used for clonal analysis was based on restriction fragment length polymorphism of the X-chromosome-linked phosphoglycerokinase (PGK) gene and on the differential methylation of the PGK gene due to random inactivation of 1 of 2 X-chromosomes by methylation in females. Among 76 gynecologic tumors tested, 34 were found to be heterozygous for the BstXI polymorphism of the PGK gene. All the 25 gynecologic cancers (4 cervix, 11 endometrium, 7 ovary and 3 fallopian tube) and 22 uterine leiomyomas analyzed by the PCR-based method were monoclonal in origin while adjacent normal tissues were polyclonal. When DNA samples were prepared from widely separated sites of tumors and/or metastatic lesions, every sample was found to be monoclonal, and the same allele of that PGK gene was inactivated in each case. All leiomyoma samples consisted of single type of inactive allele, even though alleles were detected that were specific to each nodule indicating the leiomyoma nodules were unicellular in origin but generated independently. These results demonstrate that clonal analysis by PCR offers a good method for studying clonality in small DNA samples prepared from cryostat sections of tumors. This method could be applied to distinguish between benign and malignant gynecologic lesions.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Sawada M.et al.: "Clonal analysis of human gynecologic cancers by means of the polymerase chain reaction" International Journal of Cancer. 58. 492-496 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 澤田益臣,他: "PCR法を用いた婦人科癌のクロナリティ解析" 産科と婦人科. 61. 1449-1455 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto K.et al.: "Clonal determination of uterine leiomyomas by analyzing drfferential inactivation of the X-chromosome linked phosphoglycerokinase gene" Gynecologic and Obstetric Investigation. (Submitting).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sawada M., Azuma C., et al.: "Clonal analysis of human gynecologic cancers by means of the polymerase chain reaction" Int.J,Cancer. vol.58. 492-496 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sawada M., Azuma C., et al.: "Clonal analysis of gynecologic cancer using the polymerase chain reaction method." Sanka To Fujinka. vol.61 (In Japanese). 1449-1455 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto K,Azuma C., Sawada M., et al.: "Clonal determination of uterine leiomyomas by analyzing differential inactivation of the X-chromosome linked phosphoglycerokinase gene." Gynecol.Obstet. Inv.(submitting).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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