1994 Fiscal Year Final Research Report Summary
STUDY ON THE EXPRESSION AND THE FUNCTION OF HLA-G USING ANTI-HLA-G MONOCLONAL ANTIBODY
| Project/Area Number |
05671391
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | NARA MEDICAL UNIVERSITY |
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Principal Investigator |
ISHITANI Akiko NARA MEDICAL UNIVERSITY,DEPARTMENT OF LEGAL MEDICINE,ASSOCIATE RESEARCHER, 医学部, 助手 (40112544)
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| Project Period (FY) |
1993 – 1994
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| Keywords | HLA-G / PLACENTA / TROPHOBLAST / MONOCLONAL ANTIBODY / IMMUNOHISTOCHEMISTRY / IMMUNOSUPRESSOR / REPRODUCTIVE IMMUNOLOGY |
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| Research Abstract |
(1) Study on the distribution of HLA-G antigen in human tissues
Fourty five kinds of tissues including placenta were examined for a expression of HLA-G antigen by immunological method using monoclonal antibody specific to HLA-G,Non of them reacted with the antibody without placenta. In placent (38 samples) at all stages of fetal development, HLA-G was expressed only on cytotrophoblastic cell column, cytotrophoblastic shell and invasive cytotrophoblas but on chorionic villous cytotrophoblast and syncytiotrophoblast. These resultsfuther substantiate the assumption that the HLA-G anti-gen play an essential role in the immunological process in pregnancy.
(2) Study on a soluble HLA-G antigen
We found that the soluble antigen might be produced by the alternative splicing of the transcripts, in which the intron 4 was not spliced out resulting in excluding the transmembrane encoding region. And we also succeeded in producing monoclonal antibody specific to solubleHLA-G antigen which is used to detect the soluble antigen in placenta and cord blood.
(3) Study on proccessing of HLA-G antigen and the bound peptides
To gain insight into the processing of HLA-G and its ability to bind peptide, we undertook an examination of the expression of HLA-G in the TAP negative cell line and compared it with the expression in wild type cell line. Membrane bound HLA-G expression in the TAP negative cells is reduced to about 20%, and the soluble antigen is 5% of the levels present in the wild type cells. The membrane and soluble HLA-G proteins bind essentially the same set of peptides which are derived from a variety of intracellular proteins.
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