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1994 Fiscal Year Final Research Report Summary

Molecular biological study of the latent viral infection and its reactivation of the human sensory ganglia in the temporal bone.

Research Project

Project/Area Number 05671411
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionHokkaido University

Principal Investigator

FUKUDA Satoshi  Hokkaido University School of Medicine Medical Hospital, Lecture, 医学部・附属病院, 講師 (20125347)

Project Period (FY) 1993 – 1994
KeywordsHerpes Simplex Virus / Copy Number / Latent Viral Infection / Human Geniculate Ganglia / Human Spiral Ganglia / Human Vestibular Ganglia / Polymerase Chain Reaction (PCR) / In Situ Hybridization (ISH)
Research Abstract

Viral infection, in particular, by reactivation of latent herpes virus has been considered to be a possible explanation for the pathogenesis of idiopathic peripheral facial nerve palsy (Bell's palsy), sudden sensorineural hearing loss, vestibular neuronitis and Ramsey-Hunt syndrome. As of yet, however, latent HSV-1 and VZV in the sensory neurons of the geniculate, spiral or vestibular ganglia and the way for reactivation has been sitll unknown.
Up to now, although we could obviously detect latency-associated transcript (LAT) in trigeminal and geniculte ganglia, only one vestibular ganglia showed positive for LAT and negative in spiral ganglia. We postulated that the difference of infected copy number between these ganglia might influence these results. To elucidate this problem, in this project, we further examined these ganglia using molecular biological technique. A relative amount of LAT between each ganglia were calculated. HSV-1 DNA or copy number of LAT in trigeminal and geniculate ganglia were 10-100 times as many as taht of vbestibular ganglia. The percentage of positive hybridization number for LAT out of total ganglia calculated was 1.2% in trigeminal ganglion and 6.2% in geniculate ganglion. On the other hand, the positive rate in vestibular ganglion was under 0.1% and spiral ganglia showed no positive findings. Our results suggest the neurotropism of HSV-1 may different in each sensory ganglia, but the difference of positive infectid number for LAT between each sensory ganglia remains still nuclear.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] S.Fukuda: "The significance of herpes viral latency in the spiral ganglia." Acta Otolaryngol (Stockh) Suppl. 514. 108-110 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Fukuda: "Immunopathophisiology of the auditory system : pathophysiology of the inner ear viral infection." Pathophysiology. 1(suppl). 395- (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田中克彦: "突発性難聴とウイルス感染" Otol JPn. 4. 130-136 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furuta Y: "Latent herpes simplex virus type 1 in human vestibular ganglia." Acta Otolaryngol (Stockh) Suppl. 503. 85-89 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.suzuki: "Immunobiology in Otorhinolaryngology-Progress of a decade Latency of herpes simplex virus in the sensory ganglia of human temporal bones." Kugler Publ,Amsterdam/New York, 674 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuda S: "The significance of herpes viral latency in the spiral ganglia." Acta Otolaryngol (Stockh). Suppl 514. 108-110 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda S: "Immunopathophisiology of the auditory system ; pathophysiology of the Inner ear viral infection." Pathophysiology. 1(suppl). 395 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka K: "Sudden deafness and viral infection." Otol Jpn. 4. 130-136 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furuta Y: "Latent herpes simplex virus type 1 in human vestibular ganglia." Acta Otolaryngol (Stockh). Suppl 503. 85-89 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Skuzuki S: Latency of herpes simplex virus in the sensory ganglia of human temporal bones. in immunobiology in Otorhinolaryngology-Progress of a decade-. Kugler Publ, Amsterdam/New York, 293-296 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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