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1995 Fiscal Year Final Research Report Summary

PREVENTION OF HYPERSENSITIVITY ON RESPIRATORY TRACT BY SUPPRESSING CHEMOTACTIC ACIVITIES OF MAST CELLS

Research Project

Project/Area Number 05671445
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionTHE JIKEI UNIVERSITY SCHOLL OF MEDICINE

Principal Investigator

IMI Toru  THE JIKEI UNIVERSITY SCHOOL OF MEDICINE,OTORHINOLARYNGOLOGY,LECTURER, 医学部, 講師 (00130146)

Co-Investigator(Kenkyū-buntansha) SANEYOSHI Kensaku  THE JIKEI UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (60246410)
NOHARA Osamu  THE JIKEI UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (20237861)
KATAYAMA Noboru  THE JIKEI UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (60260949)
Project Period (FY) 1993 – 1995
KeywordsMast cell / Chemotaxis / IL-4 / Cytokine / Chemokine / Hypersensitivity / Mouse / Lymphocyte
Research Abstract

We obtained mouse cultured mast cell lines from bone marrow cells of BALB/c mice and used these lines for the experiments of mast cell chemotaxis. These lines showed several amount of histamine content and had several chemotactic activities with increasing maturation periods. The supernatant of T cell lines from mice which were immunized with cedar pollens secreted high amount of IL-4 and reacted as a chemotactant for cultured mast cells. when cultured mast cells were activated, they increased chemotactic activities and producted IL-4 10 times higher than T cell lines. In contrast, the use of steroid and treatment with heat stress suppressed chemotactic activities of mast cells. These results indicated that IL-4 production may cause local hypersensitivity during allergic reaction and the therapy should be useful to prevent it.
To know the activity as a chemotactant of T cell products in human, we stimulated peripheral T cells from the patients suffering from cedar pollinosis. The T cells from patients proliferated markedly with purified cedar pollens and increased to pruduce IL-4 but decreased to secrete IL-gamma. The immunotherapy suppressed the proliferation of T cells and production of IL-4. The culture of mast cell lines were not established from umbilical cord blood.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 今井透: "マウス培養肥満細胞の走化性" アレルギー. 42. 1431- (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野原修: "スギ花粉からの抗原物質溶出に及ぼす鼻汁の影響" アレルギー. 42. 1258- (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤巻秀和: "ディーゼル排気粒子と抗原の気管内投与によるIL-4産生の亢進" アレルギー. 42. 1449- (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今井透: "スギ特異的減感作療法によるリンパ球応答性の低下" アレルギー. 43. 1110- (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 斎藤三郎: "精製スギ花粉抗原に対するT細胞の反応性とIgE応答性" アレルギー. 43. 1109- (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今井透: "マウスT細胞を用いた、治療用スギ花粉エキスの検定の試み" アレルギー. 44. 424- (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Imai et al.: "In vitro translation of mRNA from rat peritoneal and intestinal mucosal mast cells." Int Archs Allegy Immunol. 102. 26-32 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Nakayama et al.: "Attempt to isolate mast cell precursors based on the differential sensitivity to UV-B and X-iradiation." Toxix Substance J. 13. 86-95 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Fujimaki et al.: "Changes in the response of lung mast cells isolated from rats and guinea pigs exposed to nitrogen dioxide." Inhal Toxicol. 6. 515-520 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Fujimaki et al.: "Intranasal instillation of diesel exhaust particulates and antigen in mice modulated cytokine production in cervical lymph node cells." Int Arch Allergy Immunol. 108. 268-273 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Imai: "The cases of pollinosis who were treated with anti-allergic drugs." Chiryogaku. 29. 98-100 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Imai: "The efficacy of the warm earosol therapy for pollinosis." Rinsho To Yakuzai Chiryou. 14. 239-241 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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