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1994 Fiscal Year Final Research Report Summary

Regulation of salivary secretion : dynamic analysis by confocal laser microscopy

Research Project

Project/Area Number 05671514
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionKitasato University School of Medicine

Principal Investigator

SEGAWA Akihisa  Kitasato University, School of Medicine, Lecturer, 医学部, 講師 (50154638)

Project Period (FY) 1993 – 1994
KeywordsSalivary gland / Secretion / Intracellular signaling / Confocal microscopy
Research Abstract

Rat parotid acinar cells possess two secretory systems regulated by different receptor and signal transduction mechanisms. Stimulation of the beta-adrenoceptor leads to enzyme release by exocytosis via cyclic AMP,while stimulation of alpha-adrenoceptors and muscarinic cholinoceptors triggers fluid secretion mediated by phosphoinositides and calcium. Confocal microscopy of living parotid acinar cells stimulated with the beta-agonist isoproterenol and the muscarinic agonist carbachol have shown two exocytosis mechanisms. Isoproterenol evoked exocytosis involving neither the gross enlargement of luminal membrane nor the light microscopically detectable endocytosis. Both of these membrane events were instead observed in cells stimulated with carbachol. The use of phorbol ester TPA,Thapsigargin, A23187 and H-7 in conjunction with the F-actin staining has suggested that microfilaments and calcium, but not protein kinase C,are important regulators for the discrimination of these processes.
It was also obeserved that under the secretory phase the tight junctional permeability in acini increase to allow permeation of large molecules through the paracellular pathway with flexible size-selectivity characteristics.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Akihisa Segawa: "Tight junctional permeability in living cells;dynamic changes directly visualized by confocal laser microscopy" Journal of Electron Microscopy. 43. 290-298 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 瀬川 彰久: "正常唾液腺の構造と発生" 歯科ジャーナル. 40. 783-792 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 瀬川 彰久: "口は何のためにあるか" 大森 誠(風人社), 267 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 瀬川 彰久: "新しい光学顕微鏡第二巻共焦点レーザー顕微鏡の医学・生物学への応用" 佐藤武雄(学際企画)(印刷中),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihisa Segawa: "Tight junctional permeability in living cells : Dynamic changes directly visualized by confocal laser microscopy." Jounal of Electron Microscopy. 43. 290-298 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akihisa Segawa: "Structure and development of normal salivary glands." Shika journal. 40. 783-792 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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