1994 Fiscal Year Final Research Report Summary
Effect of chemotherapeutic agent on leukocyte function
| Project/Area Number |
05671563
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
SHINOHARA Mitsuko Osaka Dent.Univ.Dept.of Pharmacol.Associate Professor, 歯学部, 助教授 (40067187)
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| Co-Investigator(Kenkyū-buntansha) |
OGATA Keigo Osaka Dent.Univ.Dept.of Pharmacol.Assistant, 歯学部, 助手 (30214000)
OHURA Kiyoshi Osaka Dent.Univ.Dept.of Pharmacol.Proffesor, 歯学部, 教授 (20131378)
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| Project Period (FY) |
1993 – 1994
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| Keywords | chemotherapeutic agent / leukocyte / macrophage / rat / chemotaxis / phagocytosis / antibiotic |
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| Research Abstract |
The effect of seven kinds of antibiotics, ampicillin (ABPC), cephalexin (CEX), cefotiam (CTM), amikacin (AMK), clindamycin (CLDM), tetracyline (TC) and bleomycin (BLM )on the rat macrophage (MPHI) chemotaxis to zymosan activated serum (ZAS) was examined. The chemotaxis of rat peritoneal MPHI to ZAS was measured by the membrane filter method using 48-well microchemotaxis chambers and then the rat MPHIs were incubated with each antibiotic. AII antibiotics dose-dependently suppressed the rat MPHI chemotaxis. At 1 mug/ml all the antibiotics, except ABPC and CEX,inhibited rat MPHI chemotaxis to ZAS significantly (CLDM : p<0.05, AMK : p<0.01, CTM,TC and BLM : p<0.001). At 10mug/ml, the antibiotics inhibited rat MPHI chemotaxis to ZAS significantly (ABPC and CLDM : p<0.01, other antibiotics : p<0.001). At 100mug/ml, the antibiotics markedly suppressed rat MPHI chemotaxis (p<0.001). All antibiotics except ABPC showed dose-dependent decrease of both phagocytosis ratio and index at doses of 10-1000mug/ml or more.CTM,CEX,TC,AMK and CLDM showed significant decrease of phagocytosis ratio (p<0.001) at the dose of 100-1000mug/ml or more. Significant decrease of phagocytosis index were shown in CTM,CEX and CLDM (p<0.01 : 1000mug/ml). TC (p<0.001 : 1000mug/ml) and AMK (p<0.01 : 100mug/ml and p<0.001 : 1000mug/ml). BLM showed significant decrease of phagocytosis ratio (p<0.001 : 10-1000mug/ml) and phagocytosis index (p<0.01 : 10mug/ml, p<0.001 : 100-1000mug/ml). ABPC slightly showed dose-dependent increase of both phagocytosis ratio and index, but was not significantly different from those of controls. These findings suggest that the accumulation of MPHIs by infection or immunological host defense is maintained by the inhibition of MPHI chemotaxis and phagocytosis of antibiotics and that this function promotes the healing of infecion.
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