1995 Fiscal Year Annual Research Report

口腔発癌モデルを用いた口腔癌発生の分子病理学的追求とその化学予防の基礎的研究

Research Project

Project/Area Number	05671568
Research Institution	Gifu University School of Medicine
Principal Investigator	田中卓二岐阜大学, 医学部, 助教授 (40126743)
Co-Investigator(Kenkyū-buntansha)	森秀樹岐阜大学, 医学部, 教授 (70021433) 吉見直己岐阜大学, 医学部, 講師 (30166996) 杉江茂幸岐阜大学, 医学部附属動物実験施設, 助教授 (60187648)
Keywords	4-nitroquinoline 1-oxide / 口腔発癌 / プロトカテク酸 / プログレッション / 化学予防 / ラット
Research Abstract	これまで4-Nitroquinoline 1-oxide(4-NQO)誘発ラット口腔発癌モデルを用いて、このモデルにおける口腔癌発生の分子病理学的背景の検討と柑橘類等に存在し抗酸化作用を有するプロトカテク酸(PCA)を含むいくつかの化合物の口腔発癌抑制作用を報告してきた。本年度は、口腔発癌プログレッションモデルの確立とPCAのプログレッション抑制作用の有無を検討した。 <材料と方法> 雄F344ラット(6週齢)88匹を5群に分け以下の処置を行った。第1群:4-NQO(20ppm)を4週間飲水投与。第2群:第1群の処置に加えて、実験開始後18週より2週間のサイクルで4-NQO(20ppm)を計4回飲水投与。第3群:第2群の処置に加えて実験開始後16週より16週間0.2%PCAを混餌投与。第4群:0.2%PCAのみを16週間混餌投与。第5群:無処置対照群。実験は32週で終了し、剖検後口腔を含む諸臓器の病理学的検索を行った。 <結果> 口腔(舌)腫瘍は第1-3群に発生し、乳頭腫・扁平上皮癌の発生頻度は第1-3群でそれぞれ16%・0%、33%・47%、10%・10%(1匹に肺転移)で、第2群、第3群の扁平上皮癌の頻度はそれぞれ第1群、第2群に比し有意に増加、低下していた。 <結論> 以上の結果より、本モデルは口腔癌のプログレッションモデルとして有用であること、またPCAはそのプログレッションを抑制することが示唆された。

Research Products

(12 results)

All Other

All Publications (12 results)

[Publications] Toshihiro Kojima, Takuji Tanaka, et al.: "Chemoprevention of spontaneous endometrial cancer in female Donryu rats by dietary indole-3-carbinol" Cancer Research. 54. 1446-1449 (1994)
[Publications] Takuji Tanaka: "Chemoprevention of oral carcinogenesis" Oral Oncology, Eurpean Journal of Cancer. 31B. 3-15 (1995)
[Publications] Takuji Tanaka, et al.: "Chemoprevention of digestive organs carcinogenesis by natural product protocatechuic acid" Cancer. 75. 1433-1439 (1995)
[Publications] Masumi Suzui, Naoki Yoshimi, Takuji Tanaka, et al.: "Infrequent H-ras mutations and absence of K-ras or p53 mutations in 4-nitroauinoline 1-oxide-induced rat tongue lesions: a multistage oral cance model for human oral squamous cell carcinomas" Molecular Carcinogenesis. 14. 294-298 (1995)
[Publications] Takuji Tanaka, et al.: "Chemoprevention of N-methyl-N-nitrosourea-induced rat glandular stomach carcinogenesis by natural product protocatechuic acid" Cancer Journal. 8. 150-156 (1995)
[Publications] Takuji Tanaka, et al.: "Inhibition of oral carcinogenesis by the arotinoid mofarotene (Ro 40-8757) in male F344 rats" Carcinogenesis. 16. 1903-1907 (1995)
[Publications] Hiroki Makita, Takuji Tanaka, et al.: "Inhibition of 4-nitroquinoline 1-oxide-induced rat oral carcinogenesis by dietary exposure of a new retinoidal butenolide,KYN-54, during the initiation and postinitiation phases" Carcinogenesis. 16. 2171-2176 (1995)
[Publications] Takuji Tanaka, et al.: "Chemoprevention of rat oral carcinogenesis by naturally occurring xanthophylls astaxanthin and canthaxanthin" Cancer Research. 55. 4059-4064 (1995)
[Publications] Takuji Tanaka, et al.: "Suppression of azoxymethane-induced rat colon carcinogenesis by dietary administration of naturally occurring xanthophylls staxanthin and canthaxanthin during the postinitiation phase" Carcinogenesis. 16. 2957-2963 (1995)
[Publications] Masami Ohnishi, Takuji Tanaka, et al.: "Chemopreventive effect of a xanthine oxidase inhibitor 1′-acetoxychavicol acetate on rat oral carcinogenesis" Japaness Journal of Cancer Research. 87(in press). (1996)
[Publications] Takuji Tanaka, et al.: "Oral Oncology, Volume IVB: Fundamental" Varma AK and Mori H, 380 (1995)
[Publications] Takuji Tanaka, et al.: "Proceedings of International Conference on Food Factors-Chemistry and Cancer Prevention-" Osawa T, et al. (in press), (1996)

1995 Fiscal Year Annual Research Report

口腔発癌モデルを用いた口腔癌発生の分子病理学的追求とその化学予防の基礎的研究

Principal Investigator

田中 卓二 岐阜大学, 医学部, 助教授 (40126743)

Research Products

[Publications] Toshihiro Kojima, Takuji Tanaka, et al.: "Chemoprevention of spontaneous endometrial cancer in female Donryu rats by dietary indole-3-carbinol" Cancer Research. 54. 1446-1449 (1994)

[Publications] Takuji Tanaka: "Chemoprevention of oral carcinogenesis" Oral Oncology, Eurpean Journal of Cancer. 31B. 3-15 (1995)

[Publications] Takuji Tanaka, et al.: "Chemoprevention of digestive organs carcinogenesis by natural product protocatechuic acid" Cancer. 75. 1433-1439 (1995)

[Publications] Takuji Tanaka, et al.: "Chemoprevention of N-methyl-N-nitrosourea-induced rat glandular stomach carcinogenesis by natural product protocatechuic acid" Cancer Journal. 8. 150-156 (1995)

[Publications] Takuji Tanaka, et al.: "Inhibition of oral carcinogenesis by the arotinoid mofarotene (Ro 40-8757) in male F344 rats" Carcinogenesis. 16. 1903-1907 (1995)

[Publications] Hiroki Makita, Takuji Tanaka, et al.: "Inhibition of 4-nitroquinoline 1-oxide-induced rat oral carcinogenesis by dietary exposure of a new retinoidal butenolide,KYN-54, during the initiation and postinitiation phases" Carcinogenesis. 16. 2171-2176 (1995)

[Publications] Takuji Tanaka, et al.: "Chemoprevention of rat oral carcinogenesis by naturally occurring xanthophylls astaxanthin and canthaxanthin" Cancer Research. 55. 4059-4064 (1995)

[Publications] Takuji Tanaka, et al.: "Suppression of azoxymethane-induced rat colon carcinogenesis by dietary administration of naturally occurring xanthophylls staxanthin and canthaxanthin during the postinitiation phase" Carcinogenesis. 16. 2957-2963 (1995)

[Publications] Masami Ohnishi, Takuji Tanaka, et al.: "Chemopreventive effect of a xanthine oxidase inhibitor 1′-acetoxychavicol acetate on rat oral carcinogenesis" Japaness Journal of Cancer Research. 87(in press). (1996)

[Publications] Takuji Tanaka, et al.: "Oral Oncology, Volume IVB: Fundamental" Varma AK and Mori H, 380 (1995)

[Publications] Takuji Tanaka, et al.: "Proceedings of International Conference on Food Factors-Chemistry and Cancer Prevention-" Osawa T, et al. (in press), (1996)

田中卓二岐阜大学, 医学部, 助教授 (40126743)