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1995 Fiscal Year Final Research Report Summary

STUDY ON ONCOGENE ALTERATIONS AND EXPRESSION OF ONCOGENE PRODUCTS IN THE ORAL TUMORS AND THE APPLICATION FOR CLINICAL MEDICINE

Research Project

Project/Area Number 05671690
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionNIHON UNIVERSITY

Principal Investigator

KUSAMA Kaoru  NIHON UNIV., SCH of DENT,LECTURER, 歯学部, 講師 (20130479)

Co-Investigator(Kenkyū-buntansha) ODA Yasuyuki  NIHON UNIV., SCH of DENT,ASSISTANT, 歯学部, 助手 (60214144)
KUDO Itsuro  NIHON UNIV., SCH of DENT,PROFESSOR, 歯学部, 教授 (90059147)
Project Period (FY) 1993 – 1995
Keywordsoncogene / oncoprotein / gene alteration / oral cancer / squamous cell carcinoma / ras gene / c-erbB1 / c-erbB2
Research Abstract

Expression of oncoproteins such as ras p21, EGFR and c-erbB2 protein in various kinds of oral epithelial lesions including squamous cell carcinoma (SSC) was examined by an immunohistochemical method. Weak or few reaction for c-erbB2 protein was found in the tissues of oral SSCs. On the other hand, strong reaction for ras p21 and EGFR was observed in cancer tissues and the reactivity was stronger than that in non-cancerous oral epithelial lesions. Although the presence of point mutation in codon 12 of H-ras gene in some SSC cases was suggested by Msp I digestion method, no alterations in H- and K-ras genes were identified in any cases of SSC by direct sequence and SSCP methods, Amplification of c-erbB1 was found in one third of SSC cases by Southern hybridization, which were classified into Stage I,suggesting that the alteration of c-erbB1 may be related to the early stage of oral SSC.Strong reaction for ras p21 was found in all cases of salivary gland tumor by an immunohistochemical method. In adenoid cystic carcinoma the reactivity for c-erbB2 protein was stronger than that for EGFR.Althogh the alteration of K-ras gene was suggested by TGGE in pleomorphic adenomas, no amplification of c-erbB1 and c-erbB2 was detected in adenoid cystic carcinomas by Southern hybridization. In addition to these results, enhanced expression of p53 protein and tha alteration of p53 gene were observed in one third of epithelial dysplasias and oral SSCs. This result suggests that p53 gene mutation may be involved in carcinogenesis in the oral squamous epithelium even in the early stage of the dysplasia-carcinoma sequence.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 鈴木円 (指導:工藤逸郎): "口腔扁平上皮癌におけるras p21およびras遺伝子に関する研究" 日本歯学. 67. 722-731 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okutsu S.: "Expression of ras p21 and ras gene alteration in pleomorphic adenomas." J.Nihon Univ.Sch.Dent.35. 200-203 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 草間薫: "口腔扁平上皮癌の発生、増殖および進展におけるp53蛋白およびp53遺伝子について" 消化器と免疫. 29. 223-228 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹田秋生 (指導:工藤逸郎): "口腔領域の悪性腫瘍におけるEGFR,c-erbB-2蛋白およびそれらの遺伝子に関する研究" 日本歯学. 69. 434-446 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusama K.: "p53 gene alterations and p53 protein in oral epithelial dysplasia and squamous cell carcinoma." J.Pathol.178(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusama K.: "Oral Oncology Vol.IV B" Varma A.K.,Mori M.Macmillan India Ltd, 380 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusama K.: "Advances in Mucosal Immunology" Mesteckey J.et al.,Plenum Press, (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki T.(Director : Kudo I.): "A study of ras p21 and ras genes in oral squamous cell carcinoma" Nihon Univ.Dent.J.67. 722-731 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okutsu S.et al: "Expression of ras p21 and ras gene alterration in pleomorphic adenomas." J.Nihon Univ.Sch.Dent.35 (3). 200-203 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeda A.(Director : Kudo I.): "A study on the EGFR,c-erbB2 proteins and their genes in oral malignant tumors" Nihon Univ.Dent.J.69. 434-446 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusama K.et al: "p53 gene alterations and p53 protein in oral epithelial dysplasia and squamous cell carcinoma." J.Pathol.178 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusama K.et al.: ras p21, EGFR,p53 protein and their gene alterrations in oral epithelial lesions. Oral Oncology Vol.IV B,Varma A.K., Mori M.Macmillan India Ltd, New Delhi, 27-30 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusama K.et al.: Expression of ras p21 in salivary gland tumors. Advances in Mucosal Immunology, Mesteckey J.et al., Adv.Exp.Med.Biol.371B PT2. Plenum Press, New York, 1187-1190 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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