STUDIES ON INCREASING OF ANTI TUMOR EFFECTS BY INDUCING EXPRESSION OF CELL-ADHESION MOLECULES ON TUMOR CELLS

1995 Fiscal Year Final Research Report Summary

• Project/Area Number: 05671693
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: KANAGAWA DENTAL COLLEGE
• Principal Investigator: SINDO Junichi KANAGAWA DENTAL COLLEGE,DENTISTRY,PROFESSOR, 歯学部, 教授 (10084758)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Gouichi KANAGAWA DENTAL COLLEGE,DENTISTRY,RESEARCH ASSISTANT, 歯学部, 助手 (60199867) ; KOSE Akira KANAGAWA DENTAL COLLEGE,DENTISTRY,ASISTANT PROF., 歯学部, 講師 (70215268)
• Project Period (FY): 1993 – 1995
• Keywords: ICAM-1 / IFN-gamma / TNF-alpha / IL-2 / IFN-beta

Research Abstract

1, We investigated the effects of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) treatments of NR-S1 cells (morine squamouse cell carcinoma). Since NR-S1 cells treated by combination of IFN-gamma and TNF-alpha were increased susceptibility to conjugation and lysis by lymphokine activated killer (LAK) cells, we examined the participation of cell-adheision molecules on the NR-S1 cells. Untreated NR-S1 cells expressed MHC class I,MHC class II,intercellular-adheision molecule-1 (ICAM-1) and ICAM-2. Treatment with IFN-gamma induced the expression of MHC class I and ICAM-1 molecules, but did not affect the expression of MHC class II and ICAM-2 molecules on NR-S1 cells. Treatment with TNF-alpha induced the expression of ICAM-1 molecule, but did not affect the other molecule's expression. However, treatment with the combination of IFN-gamma and TNF-alpha induced strongly expression of MHC class I and ICAM-1 molecules, slightly expression of ICAM-2 molecule compared with IFN-gamma or TNF-alpha alone. As the results of the expression of these molecules, NR-S1 cells became to be more adheisive to LAK cells and more susceptibility lysis by LAK cells.
2, Three patients with oral cancer were treated by peritumoral injection of IL-2 and IFN-beta every day for ten days. Finised injections, enhancement of NK actitity of patient's peripheral blood lymphocytes occurred, the percentage of IL-2 receptor, CD3, CD8 and CD56 positive cells in peripheral blood lymphocytes increased.