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1994 Fiscal Year Final Research Report Summary

ANALYSIS OF INTRAFAMILIAL TRANSMISSION OF MUTANS STREPTOCOCCI USING DNA FINGERPRINTING

Research Project

Project/Area Number 05671711
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 矯正・小児・社会系歯学
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

KOZAI Katsuyuki  Hiroshima Univ.Dental Hospital ASSISTANT PROFESSOR, 歯学部・附属病院, 講師 (10178212)

Project Period (FY) 1993 – 1994
KeywordsDENTAL CARIES / DNA FINGERPRINTING / INTRAFAMILIAR TRANSMISSION / CONTACT ANGLE / MUTANS STREPTOCOCCI / GLUCOSYLTRANSFERASE / MOLECULAR BIOLOGY
Research Abstract

I studied the intrafamilial transmission of mutans streptococci using DNA fingerprinting.
1.Number of strain of mutans streptococci in oral cavity.
The discrimination of homology of 700 strains of mutans streptococci separated from 35 persons in 9 families revealed that one to three species inhabit in oral cavity in many subjects. Approximately 48.6% of subjects have two species of mutans streptococci.
2.The molecular biological proof concerning that mother is predominant mediator of transmission of mutans streptococci.
Approximatelly 82.3% of children (14 children of 17) had the same strain found in their mothers. In these children three children had all of the same strains found in mothers. The strains of five children were transmitted from only their mothers and fathers. The homology of strains of seven children were not found in their parents. The strains of three children were the same of the strains of their fathers. These results show that strains of mutans streptococci in children were transmitted from their families. especially their mother at high probability.
3.The biochemical specificity of mutans streptococci concerning of transmission
The amount of synthesis of insoluble glucan in transmitted mutans streptococci was 1.10 to 2,47 more than untransmitted strains. The proposions of insoluble glucan contained in the synthesized glucan were higher in the transmitted strains at 79.1 to 90.5% than that of untransmitted strains indicated at 18.4 to 88.7%. The values of contact angle, shows hydrophobicity of bacteria, indicated smaller in all the transmitted strains below 14゚ than other strains. These findings revealed that the transmission of mutans streptococci should concern the adherent factors of insoluble glucan synthesis or contact angle.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 中山隆介: "DNA解析によるミュータンスレンサ球菌の家族内分布に関する研究" 小児歯誌. 31. 243-1 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中山隆介: "ミュータンスレンサ球菌の家族内伝播に関する研究" 広大歯誌. 26. 389-1 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ryusuke Nakayama: "Study of Intrafaniliar Contribution of mutans streptococci using DNA fingerprinting" Jpn J.Pediatric Dent.31. 243 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Rusuke Nakayama: "The Analysis of Intrafamiliar Transmission of Mutans Streptococci" J.Hiroshima Univ.Dent.Soc.26. 389 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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