1994 Fiscal Year Final Research Report Summary
SEARCH FOR GLUTAMATE AGONISTS AND ANTAGONINSTS FROM BASIDOMYCETES
| Project/Area Number |
05671739
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
FUSHIYA Shinji TOHOKU UNIVERSITY PHARMACEUTICAL INSTITUTE ASSOCIATE PROFESSOR, 薬学部, 助教授 (80108563)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Basidiomycetes / Amanita pantherina / Clitocybe acromelalga / glutamate receptors / NMDA receptor / neuroexciting activity / acidic amino acids / opines |
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| Research Abstract |
Neurologically active amino acid derivatives were isolated from toxic mushrooms, Amanita panthrina and Clitocybe acromelalga.
1) Two amino acids showing an antagonistic activity toward NMDA receptor subtype were isolated from A.pantherina and determined to be (2R,1'R) -and (2R,1'S) -2-amino-3 (1,2-dicarboxy ethyl-thio) propanoic acids (ADPA). Four stereoisomers of ADPA were synthesized from fumaric acid and L- (or D-) cysteine to evaluate for the activity on NMDA receptor. They showed almost the same magnitude of activity.
2) Acromelic acids D and E,analogs of potent agonists of glutamic acid, were isolated from C.acromelalga. L-Stizolobic acid and L-stizolobinic acid, biosynthetic precursors of acromelic acids and antagonists of quisqualic receptor, were isolated from C.acromelalga. beta-Cyano-L-alanine and its gamma-glutamylpeptide, the neurolathyrogens of Vicia plants, were also isolated. The characteristic syndromes caused by ingestion of this mushroom might be attributed to these neurologically active constituents.
Three amino acids, valinopine, epi-leucinopine and isoleucinopine belonging to the opine family were also isolated from C.acromelalga.
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