| Research Abstract |
1. Synthesis of highly sensitive dienophiles
Fluorescent or chemiluminescent dienophiles targeting conjugated diene were designed and synthesized. A highly sensitive chemiluminescent dienophile, in which a chemiluminescent chromophore (acridinium ester group) and a reactive 1,2,4-triazoline-3,5-dione group were connected, was newly synthesized.
2.A fluorescent dienophile (DMEQ-TAD) was applied to the fluorometric analysis of vitamin D,vitamin A and microcystins.
(1) Fluorometric analysis of plasma vitamin D metabolites was examined and new accurate methods for assaying 25-OHD_3,24,25-(OH)_2D_3 and an active hormone 1,25-(OH)_2D_3 in human plasma were successfully developed.
(2) Reactions of DMEQ-TED with retinoic acid and its geometrical isomers having a pentaene system were examined. Except for the 9Z-isomer, reactions gave the mono-adducts in high selectivity while the 9Z-isomer gave only the bis-adducts indicating that DMEQ-TAD might be useful as a fluorescence labeling for retinoic acids in biological fluid.
(3) DMEQ-TAD reacted with microcystins, a cyclic heptaptide toxin from blue-green algae, at the conjugated diene part to yield the cycloadducts. The fluorometric method for assaying enviromental microcystins is under investigation.
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