1994 Fiscal Year Final Research Report Summary
DNA Profiling for identification and discrimination of crude drugs
| Project/Area Number |
05671756
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
MIZUKAMI Hajime Nagoya City University.Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (30128219)
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| Project Period (FY) |
1993 – 1994
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| Keywords | DNA profiling / Reatriction fragment Length Polymorphism / PCR / Medicinal Plant / Crude drug identification |
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| Research Abstract |
Correct identification of medicinal plants and crude drugs is prereauisite for chemical as well as pharmacological researches on natural products, and also for clinical application of traditional herbal medicines.
DNA profiling (DNA-based polymorphism assay) has several significant advantages as an approach to identifying or discriminating living organisms over morphological, chemical and biochemical approacges ; (1) the genotype rather than the phenotype is directly assayd, (2) it is not affected by organs or tissues used, developmental stages or environmental conditions, and it can be applied to rare and endangered species and dried samples such as herbarium specimens and crude drugs because DNA can be prepared from a small amount of tissues and is relatively stable.
In this reserach project I showed successful applications of RFLP (Restriction Fragment Length Polymorphism ; variation in the number and size of fragments produced by digeston of DNA with restriction endonucleases) analysis to identification of inter-specific hybrids of Dubouisia, source plants for tropane alkaloidas such as atropine and scopolamine, and analysis of geographic variation in Bupleurum falcatum and inttra-and interspecific variation of Atractylodes plants, using ribosomal DNA as a probe. Amplification of 5S-rDNA spacer regions from crude drugs and analysis of their base sequences were also investigated to show the possibility of DNA profiling for crude drug identification.
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