1994 Fiscal Year Final Research Report Summary
Biochemical and Pharmacolosical study of the Accumulation of Eycitatory amino Acids in to Synaptic Vesicles.
| Project/Area Number |
05671821
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
MORIYAMA Yoshinori Osaka University Institute of Scientific and Industrial Research Research Associate, 産業化学研究所, 助手 (10150658)
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| Project Period (FY) |
1993 – 1994
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| Keywords | Glutamate / Synaptic vesicle / Transmitter / Vacuolar ATPase / Newron / Transporter |
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| Research Abstract |
Synaptic vesicles are storage subcelluar organelles for neurotransmitters in presynaptic nerve terminals and possess at least four different kinds of transporters for neurotransmitters : One of them, a glutamate transporter is very important transporter, because glutamate is an exicitatory amino acid transmitter. So far little is known about the glutamate transporter. In this study I investigated several aspects of the transporter : (1) purification, (2) specific inhibitors, (3) biochemical study on vacuolar ATPase as a primary pump for glutamate transport. During the past two years, I succeeded at least in part in carrying out the above projects : I established the procedure for solubilization, reconstitution and partial purification of glutamate transporter. I found that dibezylglutamate as a potent competitive inhibitor for the transporter. I determinied that there are binding sites for omeprazole and quinacrine mustard in subunit A of chromaffin granules vacuolar ATPase. By cross-linking technique, I obtained several useful information on subunit/subunit interaction of vacuolar ATPases. These results are important for understanding entire feature of vesicular glutamate transporter.
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[Publications] Hong, R-M., Mori, H., Fukui, T., Moriyama, Y., Futai, M., Yamamoto, A., Tashiro, Y., and Tagaya, M.: "Association of N-ethylmaleimide-sensitive factor with synaptic vesicles." FEBS Letter. 350. 253-257 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Deguchi, J., Yamamoto, A., Yoshimori, T., Sugasawa, K., Moriyama, Y., Futai, M., Suzuki, T., Kato, K., Uyama, M., and Tashiro, Y.: "Acidification of phagosomes and degradation of rod segments in rat retinal pigment epthelium." Invest.Ophthalomol.Vis.Sci.35. 568-579 (1993)
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「研究成果報告書概要(欧文)」より
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